Efeitos do hipotireoidismo na matriz extracelular das pregas vocais de ratas adultas
Data
2024-10-08
Autores
Mororó, Welber Chaves 
Orientadores
De Biase, Noemi Grigoletto
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: Avaliar as alterações na matriz extracelular das pregas vocais de ratas adultas após a indução de hipotireoidismo. Métodos: Ratas adultas foram divididas em dois grupos: hipotireoideas (submetidas à tireoidectomia e tratamento com metimazol) e eutireoideas (controle). Após um período de 8 semanas de experimento, os animais foram submetidos à eutanásia e as pregas vocais foram coletadas. Estas foram analisadas quanto à presença de colágeno tipo I, colágeno tipo III e elastina utilizando teste imunoenzimático. E para quantificação de ácido hialurônico foi empregado método fluorométrico de detecção direta em fluídos biológicos. Resultados: A concentração de colágeno tipo I nas pregas vocais dos animais do grupo com hipotireoidismo foi superior à do grupo controle (p<0,05), sendo também observado um aumento na concentração de colageno tipo III (p<0,05). Não houve diferença nas concentrações de elastina e ácido hialurônico entre os grupos (p>0,05). No entando, duas amostras com valores discrepantes na concentração de ácido hialurônico do grupo hipotireoideo chamaram atenção devido ao seu alto valor. Conclusões: A exposição ao hipotireoidismo foi capaz de causar alterações na matriz extracelular das pregas vocais de ratas adultas. Observou-se o aumento na concentração de colágeno tipo I e tipo III nos animais com hipotireoidismo, e que não houve diferença na concentração de elastina e ácido hialurônico entre o grupo controle e hipotireoideo.
Objective: To evaluate the changes in the extracellular matrix of the vocal folds of adult female rats after induction of hypothyroidism. Methods: Adult female rats were divided into two groups: hypothyroid (after thyroidectomy and treatment with methimazole) and euthyroid (control). After an 8-week experiment, the animals were euthanized and their vocal folds were collected. These were analyzed for the presence of collagen type I, collagen type III and elastin using an enzyme immunoassay. A fluorometric method for direct detection in biological fluids was used to quantify hyaluronic acid. Results: The concentration of type I collagen in the vocal folds of animals in the hypothyroid group was higher than in the control group (p<0.05), and an increase in the concentration of type III collagen was also observed (p<0.05). There was no difference in the concentrations of elastin and hyaluronic acid between the groups (p>0.05). However, two samples with deviating hyaluronic acid concentration in the hypothyroid group stood out due to their high value. Conclusions: Hypothyroidism exposure was able to cause changes in the extracellular matrix of the vocal folds of adult female rats. The concentration of type I and type III collagen was increased in the hypothyroid animals, and there was no difference in the concentration of elastin and HA between the control and hypothyroid groups.
Objective: To evaluate the changes in the extracellular matrix of the vocal folds of adult female rats after induction of hypothyroidism. Methods: Adult female rats were divided into two groups: hypothyroid (after thyroidectomy and treatment with methimazole) and euthyroid (control). After an 8-week experiment, the animals were euthanized and their vocal folds were collected. These were analyzed for the presence of collagen type I, collagen type III and elastin using an enzyme immunoassay. A fluorometric method for direct detection in biological fluids was used to quantify hyaluronic acid. Results: The concentration of type I collagen in the vocal folds of animals in the hypothyroid group was higher than in the control group (p<0.05), and an increase in the concentration of type III collagen was also observed (p<0.05). There was no difference in the concentrations of elastin and hyaluronic acid between the groups (p>0.05). However, two samples with deviating hyaluronic acid concentration in the hypothyroid group stood out due to their high value. Conclusions: Hypothyroidism exposure was able to cause changes in the extracellular matrix of the vocal folds of adult female rats. The concentration of type I and type III collagen was increased in the hypothyroid animals, and there was no difference in the concentration of elastin and HA between the control and hypothyroid groups.
Descrição
Citação
MORORÓ, Welber Chaves. Efeitos do hipotireoidismo na matriz extracelular das pregas vocais de ratas adultas. 2024. 65 f. Tese (Doutorado em Otorrinolaringologia) - Escola Paulista de Medicina (EPM), Universidade Federal de São Paulo (UNIFESP). São Paulo, 2024.