Interação da Sakuranetina em filmes de Langmuir contendo lipídios insaturados
Data
2023-10-17
Autores
Souza, Matheus Lima de 
Orientadores
Caseli, Luciano
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Este projeto investigou a interação de sakuranetina, um flavonoide de promissora atividade farmacêutica, com monocamadas de fosfolipídios insaturados que serviram para mimetizar membranas celulares. Os fosfolipídios empregados foram o 2-Oleoil-1-palmitoil-sn-glicero-3-fosfocolina (POPC) e 2-Oleoil-1-palmitoil-sn-glicero-3-fosfoetanolamina (POPE). As monocamadas puras e mistas com o bioativo foram avaliadas através de experimentos de pressão superficial, microscopia no ângulo de Brewster (BAM) e espectroscopia de reflexão-absorção no infravermelho com modulação da polarização (PM-IRRAS). Os resultados permitem concluir que a sakuranetina apresenta interação com os fosfolipídios, devido a expansão dos filmes monomoleculares. O POPC sofreu redução na estabilidade e diminuição da elasticidade superficial frente a esse bioativo, provavelmente causados por rearranjos moleculares na interface. Interações são verificadas por PM-IRRAS entre o flavonoide e a porção polar e apolar do POPC. A contribuição viscosa ao filme misto diminuiu, diferentes domínios de densidade foram exibidos por BAM e histerese relevante não foi identificada. O POPE sofreu pequena redução da estabilidade termodinâmica, indicando que poucos rearranjos aconteceram na monocamada. Poucas agregações vistas nas imagens do BAM estão presentes na interface. Sakuranetina também aumentou a rigidez deste lipídio, mas a monocamada permaneceu essencialmente elástica e facilmente espalhável na superfície, tal qual o primeiro lipídio. Os resultados de PM-IRRAS indicaram interações com a porção polar e apolar do POPE, o que explica a expansão da monocamada. Isto posto, os dados permitem supor que a sakuranetina foi mais bem incorporada na camada monomolecular de POPE, e que membranas com POPC experienciariam maior interação e desestabilização na presença deste flavonoide.
This project investigated the interaction of sakuranetin, a flavonoid with promising pharmaceutical activity, with monolayers of unsaturated phospholipids that served to mimic cell membranes. The phospholipids used were 2 Oleoyl 1 palmitoyl sn glycero 3 ph osphocholine (POPC) and 2 Oleoyl 1 palmitoyl sn glycero 3 phosphoethanolamine (POPE). The pure and mixed monolayers with the bioactive were evaluated through surface pressure experiments, Brewster angle microscopy (BAM) and polarization modulated infrared reflection absorption spectroscopy (PM IRRAS). The results allow us to conclude that sakuranetin interacts with phospholipids, due to the expansion of monomolecular films. POPC suffered a reduction in stability and a decrease in surface elasticity compared to this bioactive, probably caused by molecular rearrangements at the interface. Interactions are verified by PM IRRAS between the flavonoid and the polar and nonpolar portion of POPC. The viscous contribution to the mixed film decreased, different densit y domains were displayed by BAM and relevant hysteresis was not identified. POPE suffered a small reduction in thermodynamic stability, indicating that few rearrangements occurred in the monolayer. Few of the aggregations seen in the BAM images are present at the interface. Sakuranetin also increased the rigidity of this lipid, but the monolayer remained essentially elastic and easily spreadable on the surface, just like the first lipid. The PM IRRAS results indicated interactions with the polar and nonpola r portion of POPE, which explains the expansion of the monolayer. That said, the data allow us to assume that sakuranetin was better incorporated into the POPE monomolecular layer, and that membranes with POPC would experience greater interaction and desta bilization in the presence of this flavonoid.
This project investigated the interaction of sakuranetin, a flavonoid with promising pharmaceutical activity, with monolayers of unsaturated phospholipids that served to mimic cell membranes. The phospholipids used were 2 Oleoyl 1 palmitoyl sn glycero 3 ph osphocholine (POPC) and 2 Oleoyl 1 palmitoyl sn glycero 3 phosphoethanolamine (POPE). The pure and mixed monolayers with the bioactive were evaluated through surface pressure experiments, Brewster angle microscopy (BAM) and polarization modulated infrared reflection absorption spectroscopy (PM IRRAS). The results allow us to conclude that sakuranetin interacts with phospholipids, due to the expansion of monomolecular films. POPC suffered a reduction in stability and a decrease in surface elasticity compared to this bioactive, probably caused by molecular rearrangements at the interface. Interactions are verified by PM IRRAS between the flavonoid and the polar and nonpolar portion of POPC. The viscous contribution to the mixed film decreased, different densit y domains were displayed by BAM and relevant hysteresis was not identified. POPE suffered a small reduction in thermodynamic stability, indicating that few rearrangements occurred in the monolayer. Few of the aggregations seen in the BAM images are present at the interface. Sakuranetin also increased the rigidity of this lipid, but the monolayer remained essentially elastic and easily spreadable on the surface, just like the first lipid. The PM IRRAS results indicated interactions with the polar and nonpola r portion of POPE, which explains the expansion of the monolayer. That said, the data allow us to assume that sakuranetin was better incorporated into the POPE monomolecular layer, and that membranes with POPC would experience greater interaction and desta bilization in the presence of this flavonoid.
Descrição
Citação
SOUZA, M. L. de. Interação da Sakuranetina em filmes de Langmuir contendo lipídios insaturados. 2023. 55 f. Trabalho de Conclusão de Curso (Graduação em Química Industrial) - Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, São Paulo, 2023.