Efeito antiapoptótico e antissenescência do açaí liofilizado (Euterpe Oleracea Martius) sobre as células germinativas masculinas
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Data
2024-04-15
Autores
Mumcu, Merve [UNIFESP]
Orientadores
Vilela, Vanessa Vendramini [UNIFESP]
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
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Resumo
O açaí (Euterpe oleracea Mart.) possui inúmeras propriedades benéficas à saúde e vem sendo utilizado no tratamento de enfermidades ligadas à síndrome metabólica e ao envelhecimento. O modelo experimental de indução à senescência, utilizando o tratamento de roedores com D-galactose, apresenta características indicativas de comprometimento cognitivo, motor e reprodutivo muito semelhantes àquelas observadas no envelhecimento natural em roedores e tem potencial para mimetizar o perfil de danos que caracterizam a idade paterna avançada. O presente estudo buscou verificar se o açaí protege a integridade da cromatina das células germinativas masculinas, por meio da modulação da expressão de da SIRT1 e ação anti-apoptótica, sob indução à senescência pela D-galactose por 4 semanas de tratamento. Ratos machos (n=24) foram subdivididos em 4 grupos: o grupo DG foi submetido ao tratamento com D-galactose em doses diárias de 200mg/kg de peso corpóreo, por 4 semanas; o grupo DGA recebeu, além do tratamento com a D-galactose, suplementação com 200mg/kg de açaí liofilizado diluído em água destilada, 5 vezes por semana; o grupo controle (C) e açaí (A) receberam, respectivamente, água destilada e açaí. Após os tratamentos, todos os animais foram anestesiados e os órgãos reprodutivos foram coletados e pesados. O tecido testicular foi processado para a quantificação de SIRT1, receptor de leptina, BAX e BCL-2. A quantificação das células germinativas apoptóticas e da qualidade da cromatina dos espermatozóides armazenados na cauda do epidídimo foram verificadas. O monosacarídio D-galactose apresentou acentuado aumento no número de células TUNEL-positivas e redução na qualidade da cromatina, o que foi significantemente revertido pelo açaí. Entretanto a expressão da receptor de leptina, BAX e BCL-2 testiculares, bem como a protaminação do DNA espermático, não apresentaram significância estatística, mas indicaram tendência de alteração para o grupo envelhecido (DG). Nas condições experimentais aqui propostas, a ação antienvelhecimento do açaí, via ativação de SIRT1, apresentou-se como uma tendência, porém, não foi detectada significância estatística quando o grupo suplementado com açaí (DGA) foi comparado ao grupo envelhecido (DG). Os resultados sugerem que o açaí apresenta efeito anti-apoptótico e protetor da qualidade genômica das células germinativas, provavelmente por via de ativação da SIRT1.
Açaí (Euterpe oleracea Mart.) has numerous beneficial properties for health and has been used to treat illnesses linked to metabolic syndrome and aging. The experimental model of senescence induction, using the treatment of rodents with D-galactose, presents characteristics indicative of cognitive, motor and reproductive impairment very similar to those observed in natural aging in rodents and has the potential to mimic the damage profile that characterizes advanced paternal age (APA). The present study sought to verify whether açaí protects the integrity of the chromatin of male germ cells, through modulation of SIRT1 expression and anti-apoptotic action, under senescence induction by D-galactose for 4 weeks of treatment. For this purpose, male Wistar rats were subdivided into 4 groups: the DG group underwent aging acceleration through treatment with D-galactose in daily doses of 200mg/kg of body weight, for a period of 4 weeks; the DGA group received, in addition to treatment with D-galactose, supplementation with 200mg/kg of lyophilized açaí diluted in distilled water 5 times a week; the control group (C) and açaí (A) received, respectively, distilled water and açaí (200 mg/kg), also for a period of 4 weeks. After the complete period of treatment, all animals were anesthetized by intraperitoneal injection of Dopalen®/Anasedan® solution and submitted to laparotomy to collect the reproductive organs, which were immediately weighed. Testicular tissue was processed for quantification of SIRT1, leptin receptor, BAX and BCL-2. Quantification of apoptotic germ cells and chromatin quality of spermatozoa stored in the cauda epididymis were verified. The monosaccharide D-galactose showed a marked increase in the number of TUNEL-positive cells and a reduction in chromatin quality, which was significantly reversed by açaí. However, the expression of leptin receptor, testicular BAX and BCL-2, as well as sperm DNA protamination, did not show statistical significance, but indicated a tendency for change for the aged group (DG). In the experimental conditions proposed here, the anti-aging action of açaí, via activation of SIRT1, appeared as a trend, however, no statistical significance was detected when the group supplemented with açaí (DGA) was compared to the aged group (DG). The results suggest that açaí has an anti-apoptotic and protective effect on the genomic quality of germ cells, probably through the activation of SIRT1.
Açaí (Euterpe oleracea Mart.) has numerous beneficial properties for health and has been used to treat illnesses linked to metabolic syndrome and aging. The experimental model of senescence induction, using the treatment of rodents with D-galactose, presents characteristics indicative of cognitive, motor and reproductive impairment very similar to those observed in natural aging in rodents and has the potential to mimic the damage profile that characterizes advanced paternal age (APA). The present study sought to verify whether açaí protects the integrity of the chromatin of male germ cells, through modulation of SIRT1 expression and anti-apoptotic action, under senescence induction by D-galactose for 4 weeks of treatment. For this purpose, male Wistar rats were subdivided into 4 groups: the DG group underwent aging acceleration through treatment with D-galactose in daily doses of 200mg/kg of body weight, for a period of 4 weeks; the DGA group received, in addition to treatment with D-galactose, supplementation with 200mg/kg of lyophilized açaí diluted in distilled water 5 times a week; the control group (C) and açaí (A) received, respectively, distilled water and açaí (200 mg/kg), also for a period of 4 weeks. After the complete period of treatment, all animals were anesthetized by intraperitoneal injection of Dopalen®/Anasedan® solution and submitted to laparotomy to collect the reproductive organs, which were immediately weighed. Testicular tissue was processed for quantification of SIRT1, leptin receptor, BAX and BCL-2. Quantification of apoptotic germ cells and chromatin quality of spermatozoa stored in the cauda epididymis were verified. The monosaccharide D-galactose showed a marked increase in the number of TUNEL-positive cells and a reduction in chromatin quality, which was significantly reversed by açaí. However, the expression of leptin receptor, testicular BAX and BCL-2, as well as sperm DNA protamination, did not show statistical significance, but indicated a tendency for change for the aged group (DG). In the experimental conditions proposed here, the anti-aging action of açaí, via activation of SIRT1, appeared as a trend, however, no statistical significance was detected when the group supplemented with açaí (DGA) was compared to the aged group (DG). The results suggest that açaí has an anti-apoptotic and protective effect on the genomic quality of germ cells, probably through the activation of SIRT1.
Descrição
Citação
MUMCU, Merve. Efeito antiapoptótico e antissenescência do açaí liofilizado (Euterpe Oleracea Martius) sobre as células germinativas masculinas. 2024. 70 f. Dissertação (Mestrado em Biologia Estrutural e Funcional) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP). São Paulos, 2024.