The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients

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dc.contributor.authorDantas, Katia Cristina
dc.contributor.authorFreitas, Roseli S.
dc.contributor.authorMoreira, Adriana Pardini Vicentini [UNIFESP]
dc.contributor.authorSilva, Marcos Vinicius da
dc.contributor.authorBenard, Gil
dc.contributor.authorVasconcellos, Cidia
dc.contributor.authorCriado, Paulo Ricardo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionState Hlth Secretariat
dc.contributor.institutionEmilio Ribas Infectol Inst
dc.contributor.institutionInsitute State Publ Employees IAMSPE
dc.date.accessioned2018-06-15T13:46:03Z
dc.date.available2018-06-15T13:46:03Z
dc.date.issued2013-01-01
dc.description.abstractThe aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.en
dc.description.affiliationUniv Sao Paulo FMUSP, Fac Med Med, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo HCFMUSP, LIM 53, Hosp Clin, Fac Med, Sao Paulo, Brazil
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Sao Paulo, Brazil
dc.description.affiliationState Hlth Secretariat, Adolfo Lutz Inst, Sao Paulo, Brazil
dc.description.affiliationEmilio Ribas Infectol Inst, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo FMUSP, Fac Med, Dept Dermatol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo FMUSP, Fac Med, Stricto Sensu Postgrad Course, Sao Paulo, Brazil
dc.description.affiliationInsitute State Publ Employees IAMSPE, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo HCFMUSP, Fac Med, Hosp Clin, Div Dermatol, Sao Paulo, Brazil
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFaculty of Medicine of the University of Sao Paulo (FMUSP)
dc.format.extent141-143
dc.identifierhttp://dx.doi.org/10.1590/S0365-05962013000100025
dc.identifier.citationAnais Brasileiros De Dermatologia. Rio De Janeiro Rj: Soc Brasileira Dermatologia, v. 88, n. 1, p. 141-143, 2013.
dc.identifier.doi10.1590/S0365-05962013000100025
dc.identifier.fileS0365-05962013000100025.pdf
dc.identifier.issn0365-0596
dc.identifier.scieloS0365-05962013000100025
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/42508
dc.identifier.wosWOS:000316807000025
dc.language.isoeng
dc.publisherSoc Brasileira Dermatologia
dc.relation.ispartofAnais Brasileiros De Dermatologia
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAIDS-related opportunistic infectionsen
dc.subjectHistoplasmaen
dc.subjectHIV seropositivityen
dc.subjectMolecular diagnostic techniquesen
dc.subjectPolymerase chain reactionen
dc.titleThe use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patientsen
dc.title.alternativeUso da Reação em Cadeia da Polimerase aninhada (PCR aninhada) para o diagnóstico precoce da infecção pelo Histoplasma capsulatum no soro e no sangue total de pacientes HIV positivospt
dc.typeinfo:eu-repo/semantics/article
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