The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients

Dantas, Katia Cristina; Freitas, Roseli S.; Moreira, Adriana Pardini Vicentini [UNIFESP]; Silva, Marcos Vinicius da; Benard, Gil; Vasconcellos, Cidia; Criado, Paulo Ricardo

The use of nested Polymerase Chain Reaction (nested PCR) for the early diagnosis of Histoplasma capsulatum infection in serum and whole blood of HIV-positive patients

Data

2013-01-01

Autores

Dantas, Katia Cristina

Freitas, Roseli S.

Moreira, Adriana Pardini Vicentini

Silva, Marcos Vinicius da

Benard, Gil

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Tipo

Artigo

Resumo

The aim of the study was to detect the rDNA sequences and their regions in Histoplasma capsulatum, which could be considered species-specific and used as a molecular method for this diagnosis by the technique of nested polymerase chain reaction (nested PCR), employing specific sequences (primers) for H. capsulatum: 18S rDNA region (HC18), 100 kDa (HC100) and the sequence 5.8 S-ITS rDNA (HC5.8). The PCR sequences HC18, HC100 and HC5.8 resulted in a specificity of 100%. The molecular assays may increase the specificity, sensitivity and speed in the diagnosis of Histoplasmosis.

Citação

Anais Brasileiros De Dermatologia. Rio De Janeiro Rj: Soc Brasileira Dermatologia, v. 88, n. 1, p. 141-143, 2013.