Effect of varicocele on sperm function and semen oxidative stress

Página do item simplificado
dc.contributor.authorBlumer, Camile Garcia [UNIFESP]
dc.contributor.authorRestelli, Adriana Ester [UNIFESP]
dc.contributor.authorDel Giudice, Paula Toni [UNIFESP]
dc.contributor.authorSoler, Thiesa Butterby [UNIFESP]
dc.contributor.authorFraietta, Renato [UNIFESP]
dc.contributor.authorNichi, Marcilio
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.authorCedenho, Agnaldo Pereira [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T14:17:36Z
dc.date.available2016-01-24T14:17:36Z
dc.date.issued2012-01-01
dc.description.abstractOBJECTIVETo assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity.PATIENTS and METHODSIn all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin).RESULTSThe varicocele group showed fewer spermatozoa with intact DNA (grade II, P = 0.040), more cells with inactive mitochondria (class III, P = 0.001), fewer cells with active mitochondria (class I, P = 0.005) and fewer spermatozoa with intact acrosomes (P < 0.001). Finally, no significant differences were observed in lipid peroxidation levels.CONCLUSIONMen with varicocele showed an increase in sperm DNA fragmentation and a reduction in mitochondrial activity and acrosome integrity. However, lipid peroxidation levels remained unchanged.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Anim Reprod, Sch Vet Med, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04039060 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 06/58657-1
dc.format.extent259-265
dc.identifierhttp://dx.doi.org/10.1111/j.1464-410X.2011.10240.x
dc.identifier.citationBju International. Malden: Wiley-Blackwell, v. 109, n. 2, p. 259-265, 2012.
dc.identifier.doi10.1111/j.1464-410X.2011.10240.x
dc.identifier.issn1464-4096
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/34354
dc.identifier.wosWOS:000299081700025
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofBju International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectvaricoceleen
dc.subjectspermatozoaen
dc.subjectoxidative stressen
dc.subjectacrosomeen
dc.subjectDNA damageen
dc.subjectmitochondriaen
dc.subjectmetabolismen
dc.titleEffect of varicocele on sperm function and semen oxidative stressen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos