Effect of varicocele on sperm function and semen oxidative stress

Blumer, Camile Garcia [UNIFESP]; Restelli, Adriana Ester [UNIFESP]; Del Giudice, Paula Toni [UNIFESP]; Soler, Thiesa Butterby [UNIFESP]; Fraietta, Renato [UNIFESP]; Nichi, Marcilio; Bertolla, Ricardo Pimenta [UNIFESP]; Cedenho, Agnaldo Pereira [UNIFESP]

Effect of varicocele on sperm function and semen oxidative stress

Data

2012-01-01

Autores

Blumer, Camile Garcia

Restelli, Adriana Ester

Del Giudice, Paula Toni

Soler, Thiesa Butterby

Fraietta, Renato

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Artigo

Resumo

OBJECTIVETo assess the effect of varicocele on sperm DNA integrity, mitochondrial activity, lipid peroxidation and acrosome integrity.PATIENTS and METHODSIn all, 30 patients with a clinically diagnosed varicocele of grade II or III and 32 men without a varicocele were evaluated for sperm DNA fragmentation (comet assay), mitochondrial activity (3,3'-diaminobenzidine assay), lipid peroxidation (malondialdehyde) and acrosome integrity (fluorescent probe labelled peanut agglutinin).RESULTSThe varicocele group showed fewer spermatozoa with intact DNA (grade II, P = 0.040), more cells with inactive mitochondria (class III, P = 0.001), fewer cells with active mitochondria (class I, P = 0.005) and fewer spermatozoa with intact acrosomes (P < 0.001). Finally, no significant differences were observed in lipid peroxidation levels.CONCLUSIONMen with varicocele showed an increase in sperm DNA fragmentation and a reduction in mitochondrial activity and acrosome integrity. However, lipid peroxidation levels remained unchanged.

Citação

Bju International. Malden: Wiley-Blackwell, v. 109, n. 2, p. 259-265, 2012.