Polimorfismos da p53 em mulheres com e sem expressão de RNAm E6/E7 de HPV
Data
2015-12-31
Autores
Chuery, Ana Carolina Silva 
Orientadores
Speck, Neila Maria de Gois
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: avaliar a associação entre polimorfismos da p53 e expressão de RNAm E6/E7 de papilomavírus humano (HPV). Métodos: estudo transversal que analisou 175 amostras cervicais de mulheres de 16 a 69 anos de idade que realizaram o teste de RNAm E6/E7 de HPV (NucliSENS® EasyQ® HPV). As amostras foram divididas em três grupos: positivo (n=75), aquelas com expressão de RNAm E6/E7 de HPV e captura híbrida (CH) para HPV de alto risco positiva; negativo (n=52), aquelas sem expressão de RNAm E6/E7 de HPV e CH positiva; e controle (n=48), as sem expressão de RNAm E6/E7 de HPV e CH negativa. A pesquisa dos polimorfismos nos códons 11, 72 e 248 da p53 foi feita pelo método polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Resultados: a frequência de genótipo com homozigose arginina/arginina no códon 72 foi significantemente maior no grupo positivo (49,3%) do que nos grupos negativo (32,7%) e controle (20,8%, p=0,002*). A frequência do alelo arginina também foi significantemente maior no grupo positivo (67,3%) do que nos grupos negativo (53,8%) e controle (38,5%, p<0,001*). A razão de chances da homozigose arginina/arginina associar-se com expressão de RNAm E6/E7 de HPV (grupo positivo) em comparação aos outros grupos (negativo e controle) foi 2,633 vezes (intervalo de confiança de 95%: 1,399-4,954, p=0,003*). A frequência de homozigose arginina/arginina no códon 72 manteve-se significativamente mais frequente no grupo positivo em comparação aos outros grupos nas amostras de mulheres com idade ?30 anos. Não houve diferença estatisticamente significante na distribuição genotípica nem na frequência alélica dos polimorfismos nos códons 11 e 248 entre os grupos positivo, negativo e controle. Conclusão: a presença de homozigose arginina/arginina no códon 72 da p53 associou-se significantemente com expressão de RNAm E6/E7 de HPV.
Objective: To evaluate the association between the presence of p53 polymorphisms and expression of human papillomavirus (HPV) E6/E7 mRNA. Methods: cross-sectional study that analyzed 175 cervical samples of women 16 to 69 years old who underwent HPV E6/E7 mRNA testing (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n=75), those with expression of HPV E6/E7 mRNA plus positive high-risk HPV hybrid capture (HC); negative (n=52), those without expression of HPV E6/E7 mRNA but positive high-risk HPV HC; and control (n=48), those without expression of HPV E6/E7 mRNA plus negative high-risk HPV HC. The presence of p53 codons 11, 72 and 248 polymorphisms was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: for p53 codon 72 polymorphisms, the frequency of arginine/arginine homozygosis was significantly higher in positive group (49.3%) than negative (32.7%) and control groups (20.8%, p=0.002*). Also, the frequency of arginine allele was significantly higher in the positive group (67.3%) than negative (53.8%) and control groups (38.5%, p<0.001*). The odds ratio of arginine/arginine homozygote women present expression of HPV E6/E7 mRNA (positive group) compared to other women (negative and control groups) was 2.633 times (95% confidence interval: 1.399-4.954, p=0.003*). The frequency of codon 72 arginine/arginine homozygosis remained significantly higher in positive group compared to negative and control groups in women ?30 years old. There were no statistically significant differences neither in the frequency of homozygote and heterozygote genotypes nor in the allele frequency of p53 codons 11 and 248 polymorphisms among positive, negative, and control groups. Conclusion: the presence of p53 codon 72 arginine/arginine homozygosis was significantly associated to the expression of HPV E6/E7 mRNA.
Objective: To evaluate the association between the presence of p53 polymorphisms and expression of human papillomavirus (HPV) E6/E7 mRNA. Methods: cross-sectional study that analyzed 175 cervical samples of women 16 to 69 years old who underwent HPV E6/E7 mRNA testing (NucliSENS® EasyQ® HPV). The samples were divided into three groups: positive (n=75), those with expression of HPV E6/E7 mRNA plus positive high-risk HPV hybrid capture (HC); negative (n=52), those without expression of HPV E6/E7 mRNA but positive high-risk HPV HC; and control (n=48), those without expression of HPV E6/E7 mRNA plus negative high-risk HPV HC. The presence of p53 codons 11, 72 and 248 polymorphisms was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: for p53 codon 72 polymorphisms, the frequency of arginine/arginine homozygosis was significantly higher in positive group (49.3%) than negative (32.7%) and control groups (20.8%, p=0.002*). Also, the frequency of arginine allele was significantly higher in the positive group (67.3%) than negative (53.8%) and control groups (38.5%, p<0.001*). The odds ratio of arginine/arginine homozygote women present expression of HPV E6/E7 mRNA (positive group) compared to other women (negative and control groups) was 2.633 times (95% confidence interval: 1.399-4.954, p=0.003*). The frequency of codon 72 arginine/arginine homozygosis remained significantly higher in positive group compared to negative and control groups in women ?30 years old. There were no statistically significant differences neither in the frequency of homozygote and heterozygote genotypes nor in the allele frequency of p53 codons 11 and 248 polymorphisms among positive, negative, and control groups. Conclusion: the presence of p53 codon 72 arginine/arginine homozygosis was significantly associated to the expression of HPV E6/E7 mRNA.
Descrição
Citação
CHUERY, Ana Carolina Silva. Polimorfismos da p53 em mulheres com e sem expressão de RNAm E6/E7 de HPV. 2015. 91 f. Tese (Doutorado em Ginecologia) - Escola Paulista de Medicina, Universidade Federal de São Paulo (UNIFESP), São Paulo, 2015.