Chromomycin A2 Induces Autophagy in Melanoma Cells

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dc.contributor.authorGuimaraes, Larissa Alves
dc.contributor.authorJimenez, Paula Christine [UNIFESP]
dc.contributor.authorSilva Sousa, Thiciana da
dc.contributor.authorFreitas, Hozana Patricia S.
dc.contributor.authorRocha, Danilo Damasceno
dc.contributor.authorWilke, Diego Veras
dc.contributor.authorMartin, Jesus
dc.contributor.authorReyes, Fernando
dc.contributor.authorLoiola Pessoa, Otilia Deusdenia
dc.contributor.authorCosta-Lotufo, Leticia Veras
dc.contributor.institutionUniv Fed Ceara
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFdn MEDINA
dc.date.accessioned2016-01-24T14:38:13Z
dc.date.available2016-01-24T14:38:13Z
dc.date.issued2014-12-01
dc.description.abstractThe present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. the cytotoxic activity of chromomycin A2 was evaluated across a panel of human tumor cell lines, which found IC50 values in the nM-range for exposures of 48 and 72 h. MALME-3M, a metastatic melanoma cell line, showed the highest sensitivity to chromomycin A2 after 48h incubation, and was chosen as a model to investigate this potent cytotoxic effect. Treatment with chromomycin A2 at 30 nM reduced cell proliferation, but had no significant effect upon cell viability. Additionally, chromomycin A2 induced accumulation of cells in G(0)/G(1) phase of the cell cycle, with consequent reduction of S and G(2)/M and unbalanced expression of cyclins. Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. Moreover, exposure to chromomycin A2 also induced the appearance of acidic vacuolar organelles in treated cells. These features combined are suggestive of the induction of autophagy promoted by chromomycin A2, a feature not previously described for chromomycins.en
dc.description.affiliationUniv Fed Ceara, Inst Marine Sci, BR-60165081 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Physiol & Pharmacol, BR-60430270 Fortaleza, Ceara, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 São Paulo, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Organ & Inorgan Chem, BR-60021970 Fortaleza, Ceara, Brazil
dc.description.affiliationFdn MEDINA, Ctr Excelencia Invest Medicamentos Innovadores An, Granada 18016, Spain
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Marine Sci, BR-11030400 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico (FUNCAP-Programa Areas Estrategicas)
dc.format.extent5839-5855
dc.identifierhttp://dx.doi.org/10.3390/md12125839
dc.identifier.citationMarine Drugs. Basel: Mdpi Ag, v. 12, n. 12, p. 5839-5855, 2014.
dc.identifier.doi10.3390/md12125839
dc.identifier.fileWOS000346796500006.pdf
dc.identifier.issn1660-3397
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38488
dc.identifier.wosWOS:000346796500006
dc.language.isoeng
dc.publisherMdpi Ag
dc.relation.ispartofMarine Drugs
dc.rightsAcesso aberto
dc.subjectStreptomycesen
dc.subjectautophagyen
dc.subjectcytotoxicityen
dc.subjectchromomycinen
dc.subjectmarine microorganismsen
dc.subjectBrazilen
dc.titleChromomycin A2 Induces Autophagy in Melanoma Cellsen
dc.typeArtigo
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