Macrophages and apoptosis in the stellate reticulum of the rat enamel organ
Data
2000-08-01
Autores
Baratella, Liriane 
Katchburian, Eduardo
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Tooth germs of upper first molars of 1, 3, and 5-d-old rats, fixed in formaldehyde, were stained for the detection of apoptosis by the TUNEL method, and by the ate-dye method for the demonstration of acid phosphatase. for conventional light and electron microscopy the specimens were fixed in glutaraldehyde-formaldehyde and embedded in glycol methacrylate and Araldite. Results showed that macrophages, present in the stellate reticulum, contained basophilic bodies and TUNEL-positive globules, i.e. apoptotic bodies, in their interior. Macrophages also possessed strong acid phosphatase activity. Electron microscopy showed the presence of large vacuoles inside the macrophages containing dense fragmented material. Taken together these results suggest that the intra-epithelial macrophages of the stellate reticulum engulf apoptotic bodies.
Descrição
Citação
Journal of Anatomy. New York: Cambridge Univ Press, v. 197, p. 303-306, 2000.