In vitro evaluation of leptin fragments activity on the ob receptor

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dc.contributor.authorOliveira, Vani Xavier de
dc.contributor.authorFazio, Marcos Antonio [UNIFESP]
dc.contributor.authorSantos, Edson Lucas [UNIFESP]
dc.contributor.authorPesquero, Joao Bosco [UNIFESP]
dc.contributor.authorMiranda, Antonio [UNIFESP]
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T13:49:47Z
dc.date.available2016-01-24T13:49:47Z
dc.date.issued2008-05-01
dc.description.abstractIn an attempt to identify regions in the leptin molecule responsible for its bioactivity, we tested six related-leptin peptide fragments denoted: Ae-hLEP(23-47)-NH(2) (I), Ac-hLEP(48-71)-NH(2) (II), Ac-hLEP(72-88)-NH(2) (III), Ac-hLEP(92-115)-NH(2) (IV), Ac-[Ser(117)]-hLEP(116-140)-NH(2) (V), Ac-hLEP(141-164)-NH(2) (VI) and their correspondent disulfide bridged dimer forms. the activity of the fragments was evaluated in comparision to leptin, by their ability to interact with leptin receptor using a cytosensor microphysiometer. Our results indicated that the fragments IV and V and [D-Leu(4)]-OB(3) and its human sequence analog were recognized by leptin receptor present in HP-75 cells, in agreement with the results obtained by other workers, validating that this region of the molecule contain the functional epitope of the leptin molecule. Copyright (C) 2007 European Peptide Society and John Wiley & Sons, Ltd.en
dc.description.affiliationFed Univ ABC, Nat & Human Sci Ctr, Santo Andre, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biophys, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent617-625
dc.identifierhttp://dx.doi.org/10.1002/psc.957
dc.identifier.citationJournal of Peptide Science. Malden: Wiley-Blackwell, v. 14, n. 5, p. 617-625, 2008.
dc.identifier.doi10.1002/pse.957
dc.identifier.issn1075-2617
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30627
dc.identifier.wosWOS:000256065300006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Peptide Science
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectleptinen
dc.subjectsynthetic peptidesen
dc.subjectcytosensoren
dc.subjectcircular dichroismen
dc.subjectobesityen
dc.subjectrelated-leptin peptide fragmentsen
dc.titleIn vitro evaluation of leptin fragments activity on the ob receptoren
dc.typeinfo:eu-repo/semantics/article
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