In vitro evaluation of leptin fragments activity on the ob receptor
Data
2008-05-01
Autores
Fazio, Marcos Antonio 
Santos, Edson Lucas
Pesquero, Joao Bosco
Miranda, Antonio
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
In an attempt to identify regions in the leptin molecule responsible for its bioactivity, we tested six related-leptin peptide fragments denoted: Ae-hLEP(23-47)-NH(2) (I), Ac-hLEP(48-71)-NH(2) (II), Ac-hLEP(72-88)-NH(2) (III), Ac-hLEP(92-115)-NH(2) (IV), Ac-[Ser(117)]-hLEP(116-140)-NH(2) (V), Ac-hLEP(141-164)-NH(2) (VI) and their correspondent disulfide bridged dimer forms. the activity of the fragments was evaluated in comparision to leptin, by their ability to interact with leptin receptor using a cytosensor microphysiometer. Our results indicated that the fragments IV and V and [D-Leu(4)]-OB(3) and its human sequence analog were recognized by leptin receptor present in HP-75 cells, in agreement with the results obtained by other workers, validating that this region of the molecule contain the functional epitope of the leptin molecule. Copyright (C) 2007 European Peptide Society and John Wiley & Sons, Ltd.
Descrição
Citação
Journal of Peptide Science. Malden: Wiley-Blackwell, v. 14, n. 5, p. 617-625, 2008.