Desenvolvimento e avaliação de formulações tópicas contendo prebióticos e pós-bióticos frente a microrganismos patogênicos presentes na microbiota da pele com psoríase
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Data
2024-05-22
Autores
Mascarenhas, Nadine Gonçalves [UNIFESP]
Orientadores
Lopes, Patrícia Santos [UNIFESP]
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
A psoríase é uma doença inflamatória crônica, não contagiosa e imunomediada que afeta a pele. Os tratamentos disponíveis auxiliam no controle da doença, mas há limitações que interferem na eficácia e adesão aos tratamentos tópicos. Visto que a microbiota da pele é um potencial alvo terapêutico, o objetivo do estudo foi avaliar a atividade de géis à base de hidroxietilcelulose contendo prebióticos à base de β-glucanos e de pós-bióticos de Lactobacillus paracasei e Saccharomyces cerevisiae frente ao Staphylococcus aureus e Malassezia furfur in vitro, microrganismos patógenos presentes na microbiota da pele com psoríase, a fim de proporcionar novas estratégias para auxiliar no tratamento da doença e reequilíbrio da microbiota da pele. Os extratos (pós-bióticos) foram coletados durante a fase de crescimento estacionária dos probióticos. Os extratos passaram pelo processo de extração com solventes orgânicos a fim da obtenção de um perfil químico do conteúdo por meio da Cromatografia Líquida de Alta Eficiência (CLAE). As atividades dos pós-bióticos e dos β-glucanos foram avaliadas no teste de microdiluição em caldo e teste de difusão em ágar. Os géis foram avaliados no teste de difusão em ágar. A faixa de concentração entre 500 mg/ml e 125 mg/ml do extrato de L. paracasei inibiu mais de 96% o crescimento da bactéria, enquanto 500 mg/ml do extrato inibiu 89,63% o crescimento da M. furfur. A concentração de 500 mg/ml do extrato da S. cerevisiae inibiu mais de 90% o crescimento dos microrganismos. O β-glucano derivado de aveia a 0,5% (v/v) foi capaz de inibir mais de 97% do crescimento dos microrganismos, e o β-glucano derivado da levedura nas concentrações de 0,25% e 0,5% (p/v) inibiu o crescimento de S. aureus e M. furfur em 60,66% e 59,67%, respectivamente. No teste de difusão em ágar, os ativos formaram halos de inibição entre 9 mm e 15 mm frente aos microrganismos, com exceção do β-glucano derivado da levedura. Entretanto, após a incorporação dos ativos nos géis, não foi observada atividade antimicrobiana. Os resultados são promissores e mostram o potencial dos compostos em auxiliar no reequilíbrio da microbiota da pele com psoríase. São necessárias mais pesquisas para identificar as moléculas produzidas pelos probióticos, determinar os mecanismos envolvidos nessa relação e avaliar o veículo adequado para incorporação dos ativos.
Psoriasis is a chronic, non-contagious, immune-mediated inflammatory disease that affects the skin. Available treatments help control the disease, but there are limitations that interfere with adherence to topical treatments. Since the skin microbiota is a potential therapeutic target, the objective of the study was to evaluate the activity of hydroxyethylcellulose-based gels containing prebiotics based on β-glucans and postbiotics from Lactobacillus paracasei and Saccharomyces cerevisiae against Staphylococcus aureus and Malassezia furfur in vitro, pathogenic microorganisms present in the microbiota of skin with psoriasis, in order to provide new strategies to treat the disease and help rebalance the skin microbiota. Extracts (postbiotics) were collected during the stationary growth phase. The extracts went through the extraction process with organic solvents to obtain a chemical profile of the content through High Efficiency Liquid Chromatography (HPLC). The activities of postbiotics and β-glucans were evaluated in the broth microdilution test and agar diffusion test. The gels were evaluated in the agar diffusion test. The concentration range between 500 mg/ml and 125 mg/ml of the L. paracasei extract inhibited the growth of the bacteria by more than 96%, while 500 mg/ml of the extract inhibited the growth of M. furfur by 89.63%. A concentration of 500 mg/ml of the S. cerevisiae extract inhibited the growth of microorganisms by more than 90%. Oat-derived β-glucan at 0.5% was able to inhibit more than 97% of the growth of microorganisms, and yeast-derived β-glucan at concentrations of 0.25% and 0.5% inhibited the growth of S. aureus and M. furfur in 60.66% and 59.67%, respectively. In the agar diffusion test, the active ingredients formed inhibition halos between 9 mm and 15 mm against microorganisms, except for β-glucan derived from yeast. However, after incorporating the active ingredients into the gels, no antimicrobial activity was observed. The results are promising and show the potential of the compounds to help rebalance the microbiota of the skin with psoriasis. More research is needed to identify the molecules produced by probiotics, determine the mechanisms involved in this relationship and evaluate the appropriate vehicle for incorporating the active ingredients.
Psoriasis is a chronic, non-contagious, immune-mediated inflammatory disease that affects the skin. Available treatments help control the disease, but there are limitations that interfere with adherence to topical treatments. Since the skin microbiota is a potential therapeutic target, the objective of the study was to evaluate the activity of hydroxyethylcellulose-based gels containing prebiotics based on β-glucans and postbiotics from Lactobacillus paracasei and Saccharomyces cerevisiae against Staphylococcus aureus and Malassezia furfur in vitro, pathogenic microorganisms present in the microbiota of skin with psoriasis, in order to provide new strategies to treat the disease and help rebalance the skin microbiota. Extracts (postbiotics) were collected during the stationary growth phase. The extracts went through the extraction process with organic solvents to obtain a chemical profile of the content through High Efficiency Liquid Chromatography (HPLC). The activities of postbiotics and β-glucans were evaluated in the broth microdilution test and agar diffusion test. The gels were evaluated in the agar diffusion test. The concentration range between 500 mg/ml and 125 mg/ml of the L. paracasei extract inhibited the growth of the bacteria by more than 96%, while 500 mg/ml of the extract inhibited the growth of M. furfur by 89.63%. A concentration of 500 mg/ml of the S. cerevisiae extract inhibited the growth of microorganisms by more than 90%. Oat-derived β-glucan at 0.5% was able to inhibit more than 97% of the growth of microorganisms, and yeast-derived β-glucan at concentrations of 0.25% and 0.5% inhibited the growth of S. aureus and M. furfur in 60.66% and 59.67%, respectively. In the agar diffusion test, the active ingredients formed inhibition halos between 9 mm and 15 mm against microorganisms, except for β-glucan derived from yeast. However, after incorporating the active ingredients into the gels, no antimicrobial activity was observed. The results are promising and show the potential of the compounds to help rebalance the microbiota of the skin with psoriasis. More research is needed to identify the molecules produced by probiotics, determine the mechanisms involved in this relationship and evaluate the appropriate vehicle for incorporating the active ingredients.