Efeito dos probióticos Lactobacillus rhamnosus e Lactobacillus reuteri sobre o metabolismo glicêmico, respostas hormonais e fibrose cardíaca em modelo animal de estresse crônico.
Data
2024-09-05
Autores
Meneguzzi, Raissa Ribeiro Rodrigues 
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
O estresse crônico pode desencadear respostas fisiopatológicas, ativando o eixo hipotálamo-hipófise-adrenal, resultando em desequilíbrios hormonais, como o aumento dos níveis de cortisol e catecolaminas, impactando negativamente o metabolismo e o sistema cardiovascular. Especificamente, o estudo concentrou-se na relação entre o estresse crônico e o desenvolvimento de alterações metabólicas, evidenciando a hiperglicemia e possível resistência à insulina como resultados diretos dessa interação. Neste contexto, os probióticos Lactobacillus rhamnosus e Lactobacillus reuteri emergem como potenciais moduladores dessas respostas. O objetivo do presente estudo foi investigar o efeito desses probióticos em ratos Wistar submetidos ao protocolo de Estresse Crônico Moderado e Imprevisível, avaliando seus impactos nos níveis glicêmicos, hormonais (corticosterona, peptídeo C e insulina) e parâmetros histológicos cardíacos. Essa abordagem visou não apenas compreender os mecanismos fisiopatológicos subjacentes, mas também explorar estratégias terapêuticas para modular o estresse crônico e suas implicações metabólicas e cardiovasculares, proporcionando novas perspectivas para a saúde mental e física. Animais estressados apresentaram aumento da concentração circulante de corticosterona, de insulina e da deposição de colágeno no tecido cardíaco, sem efeito sobre a glicemia. O tratamento com probióticos reduziu significativamente a concentração de corticosterona, mas não mitigou totalmente o aumento da insulina circulante e a deposição de colágeno no tecido cardíaco induzidos pelo estresse crônico. Os resultados do presente estudo apontam para os efeitos deletérios do estresse crônico sobre parâmetros fisiológicos, e o potencial modulatório benéfico, mas seletivo dos probióticos sobre a secreção hormonal. Este estudo abre novas possibilidades de investigação acerca do potencial terapêutico dos probióticos no controle dos efeitos deletérios do estresse crônico.
Chronic stress can trigger pathophysiological responses by activating the hypothalamic-pituitary-adrenal axis, resulting in hormonal imbalances such as increased levels of cortisol and catecholamines, which negatively impact metabolism and the cardiovascular system. Specifically, the study focused on the relationship between chronic stress and the development of metabolic alterations, highlighting hyperglycemia and potential insulin resistance as direct outcomes of this interaction. In this context, the probiotics Lactobacillus rhamnosus and Lactobacillus reuteri emerge as potential modulators of these responses. The aim of the present study was to investigate the effect of these probiotics in Wistar rats subjected to the Chronic Mild Unpredictable Stress protocol, evaluating their impact on glycemic levels, hormonal parameters (corticosterone, C-peptide, and insulin), and cardiac histological parameters. This approach sought not only to understand the underlying pathophysiological mechanisms but also to explore therapeutic strategies to modulate chronic stress and its metabolic and cardiovascular implications, offering new perspectives for mental and physical health. Stressed animals showed an increase in circulating corticosterone and insulin levels and collagen deposition in cardiac tissue, without affecting glycemia. Probiotic treatment significantly reduced corticosterone levels but did not fully mitigate the increase in circulating insulin and the collagen deposition in cardiac tissue induced by chronic stress. The results of the present study point to the deleterious effects of chronic stress on physiological parameters and the beneficial yet selective modulatory potential of probiotics on hormonal secretion. This study thus opens new avenues for investigating the therapeutic potential of probiotics in controlling the deleterious effects of chronic stress.
Chronic stress can trigger pathophysiological responses by activating the hypothalamic-pituitary-adrenal axis, resulting in hormonal imbalances such as increased levels of cortisol and catecholamines, which negatively impact metabolism and the cardiovascular system. Specifically, the study focused on the relationship between chronic stress and the development of metabolic alterations, highlighting hyperglycemia and potential insulin resistance as direct outcomes of this interaction. In this context, the probiotics Lactobacillus rhamnosus and Lactobacillus reuteri emerge as potential modulators of these responses. The aim of the present study was to investigate the effect of these probiotics in Wistar rats subjected to the Chronic Mild Unpredictable Stress protocol, evaluating their impact on glycemic levels, hormonal parameters (corticosterone, C-peptide, and insulin), and cardiac histological parameters. This approach sought not only to understand the underlying pathophysiological mechanisms but also to explore therapeutic strategies to modulate chronic stress and its metabolic and cardiovascular implications, offering new perspectives for mental and physical health. Stressed animals showed an increase in circulating corticosterone and insulin levels and collagen deposition in cardiac tissue, without affecting glycemia. Probiotic treatment significantly reduced corticosterone levels but did not fully mitigate the increase in circulating insulin and the collagen deposition in cardiac tissue induced by chronic stress. The results of the present study point to the deleterious effects of chronic stress on physiological parameters and the beneficial yet selective modulatory potential of probiotics on hormonal secretion. This study thus opens new avenues for investigating the therapeutic potential of probiotics in controlling the deleterious effects of chronic stress.