Ultra-som e laser de baixa intensidade no reparo de lesão parcial do tendão calcâneo em ratos
Arquivos
Data
2009
Autores
Wood, Viviane Timm 
Orientadores
Hochman, Bernardo
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: O tratamento das lesões de tendão calcâneo requer longos
períodos de reabilitação. O ultra-som (US) e o laser são os recursos mais utilizados e estudados para tratar estas lesões; porém, é escasso o estudo da utilização desses agentes de forma combinada. Objetivo: Avaliar o ultrasom e o laser de baixa intensidade, no reparo de lesão parcial do tendão calcâneo em ratos. Métodos: Os 50 ratos machos Wistar tiveram seus tendões calcâneos parcialmente lesionados por um trauma direto e foram distribuidos aleatoriamente em 5 grupos: Grupo 1-Controle, 2-US, 3-laser,
4-US+ laser e 5- laser +US. Foram tratados por 5 dias consecutivos e no 6°
dia pós lesão os tendões foram removidos e avaliados quanto ao
alinhamento das fibras (birrefringência) e quantidade de colágeno tipo I e III
(picrosirius). Resultados: o grupo US apresentou melhor alinhamento das
fibras de colágenos que o grupo controle (p=0,03). Os grupos US, laser e
laser +US apresentaram maior quantidade de colágeno tipo I que o controle
(p<0,01, p=0,01 e p<0,01 respectivamente). Não houve diferença entre os
grupos tratamento entre si para nenhuma das avaliações. A avaliação para
colágenos tipo III não apresentou diferença para nenhum dos grupos.
Conclusão: O ultra-som terapêutico e a terapia laser de baixa intensidade
foram eficazes no processo de reparo de lesão parcial do tendão calcâneo em ratos. O ultra-som promoveu maior alinhamento das fibras de colágeno. O ultra-som, o laser, e a combinação laser+ultra-som aumentaram a quantidade de colágeno tipo I na região da lesão.
Background: The treatment of calcaneous tendons ruptures demands long-term rehabilitation. The ultrasound (US) and the low level laser therapy (LLLT) are the most applied and studied physical agents to treat this kind of injuries; however, only a few studies examined the combination of both physical agents. Objective: Evaluat the effects of the US and LLLT in the tendon healing in rats. Methods: Fifty male Wistar rats underwent lesion caused by direct trauma on their calcâneus tendon were randomly assigned into 5 groups: group 1 (control), group 2 (US), group 3 (LLLT), group 4 (US+LLLT) and group 5 (LLLT+US). Animals were treated for 5 consecutive days. On the 6th day after lesion, tendons were removed and assessed under polarized light microscopy. The organization of collagen fibers was detected and measured through birefringence. The collagen type predominance in the calcaneous tendons was assessed through the picrosirius analysis. Results: The group 2 (US) when compared with controls showed an increase in collagen organization (p=0.03). Groups 2 (US), 3 (LLLT) and 5 (LASER+US) revealed increase of the type I collagen synthesis when compared to control (p<0.01, p=0.01 and p<0.01, respectively). No differences were found among the treatment groups. The type III collagen did not show differences in any of the groups. Conclusion: The US and the LLLT are effective on tendon healing in rasts. The US increase collagen organization. The US, the LLLT and the combination of LLLT+US increase type I collagen synthesis.
Background: The treatment of calcaneous tendons ruptures demands long-term rehabilitation. The ultrasound (US) and the low level laser therapy (LLLT) are the most applied and studied physical agents to treat this kind of injuries; however, only a few studies examined the combination of both physical agents. Objective: Evaluat the effects of the US and LLLT in the tendon healing in rats. Methods: Fifty male Wistar rats underwent lesion caused by direct trauma on their calcâneus tendon were randomly assigned into 5 groups: group 1 (control), group 2 (US), group 3 (LLLT), group 4 (US+LLLT) and group 5 (LLLT+US). Animals were treated for 5 consecutive days. On the 6th day after lesion, tendons were removed and assessed under polarized light microscopy. The organization of collagen fibers was detected and measured through birefringence. The collagen type predominance in the calcaneous tendons was assessed through the picrosirius analysis. Results: The group 2 (US) when compared with controls showed an increase in collagen organization (p=0.03). Groups 2 (US), 3 (LLLT) and 5 (LASER+US) revealed increase of the type I collagen synthesis when compared to control (p<0.01, p=0.01 and p<0.01, respectively). No differences were found among the treatment groups. The type III collagen did not show differences in any of the groups. Conclusion: The US and the LLLT are effective on tendon healing in rasts. The US increase collagen organization. The US, the LLLT and the combination of LLLT+US increase type I collagen synthesis.
Descrição
Citação
WOOD, Viviane Timm. Ultra-som e laser de baixa intensidade no reparo de lesão parcial do tendão calcâneo em ratos. 2009. 113 f. Dissertação (Mestrado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2009.