Nitric oxide levels in cochlear fluids of guinea pig

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dc.contributor.authorYonamine, Fernando Kaoru [UNIFESP]
dc.contributor.authorOnishi, Ektor Tsuneo [UNIFESP]
dc.contributor.authorMouro, Margaret Gori [UNIFESP]
dc.contributor.authorHiga, Elisa Mieko Suemitsu [UNIFESP]
dc.contributor.authorCruz, Oswaldo Laércio Mendonça [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:45:25Z
dc.date.available2015-06-14T13:45:25Z
dc.date.issued2013-05-01
dc.description.abstractPURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Otorhinolaryngology, Head and Neck Surgery
dc.description.affiliationUNIFESP Division of Nephrology
dc.description.affiliationUNIFESP Division of Nephrology Division of Emergency Medicine and Evidence Based Medicine. Associate Researcher
dc.description.affiliationUnifespUNIFESP, Department of Otorhinolaryngology, Head and Neck Surgery
dc.description.affiliationUnifespUNIFESP, Division of Nephrology
dc.description.affiliationUnifespUNIFESP, Division of Nephrology Division of Emergency Medicine and Evidence Based Medicine. Associate Researcher
dc.description.sourceSciELO
dc.format.extent397-402
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502013000500013
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 397-402, 2013.
dc.identifier.doi10.1590/S0102-86502013000500013
dc.identifier.fileS0102-86502013000500013.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502013000500013
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/7742
dc.identifier.wosWOS:000319994200013
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectNitric Oxideen
dc.subjectCochleaen
dc.subjectGuinea Pigsen
dc.titleNitric oxide levels in cochlear fluids of guinea pigen
dc.typeinfo:eu-repo/semantics/article
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