Nitric oxide levels in cochlear fluids of guinea pig
Data
2013-05-01
Autores
Yonamine, Fernando Kaoru 
Onishi, Ektor Tsuneo
Mouro, Margaret Gori
Higa, Elisa Mieko Suemitsu
Cruz, Oswaldo Laércio Mendonça
Tipo
Artigo
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Resumo
PURPOSE: To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration. METHODS: Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280). RESULTS: Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM. CONCLUSION: It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.
Descrição
Citação
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 397-402, 2013.