Terapia a laser de baixa intensidade na reparação do tendão do calcâneo de ratos, submetido a lesão parcial
Arquivos
Data
2006
Autores
Oliveira, Flavia Schlittler 
Orientadores
Ferreira, Lydia Masako
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: O tendão calcâneo é um dos tendões mais comumente lesados e
sua cicatrização pode levar de semanas a meses para completar-se. Na busca de
acelerar o reparo tendíneo, a laserterapia de baixa intensidade tem mostrado
efeito benéfico. Objetivo: Avaliar o efeito da laserterapia de baixa intensidade
na reparação do tendão calcâneo submetido à lesão parcial, em ratos. Métodos:
Foram utilizados 60 ratos machos, distribuídos aleatoriamente em 5 grupos com
12 animais cada, sendo que 42 foram submetidos à lesão com queda de um peso
de 186 gramas de uma altura de 20cm. No Grupo 1 (controle padrão) os animais
não sofreram lesão tendínea e nem foram submetidos ao tratamento com o laser, no Grupo 2 (controle) os animais sofreram a lesão e não foram submetidos ao
tratamento com laser, nos Grupos 3, 4 e 5 os animais sofreram lesão e foram
submetidos ao tratamento com laser (830nm, P=40mW, F= 4J/cm
2
) durante 3, 5
e 7 dias, respectivamente. Os animais dos grupos 2, 3, 4 e 5 foram submetidos a
eutanásia no 8º dia após a lesão e avaliados por microscopia de polarização para
analisar o grau de organização das fibras de colágeno. Resultados: Os grupos
experimentais e o grupo controle padrão mostraram valores significativamente
maiores em relação ao alinhamento das fibras de colágeno, comparados ao
grupo controle, e não houve diferença significante quando comparados os
Grupos 1 e 4, o mesmo acontecendo entre os Grupos 3 e 5. Conclusão: A
terapia a laser de baixa intensidade é efetiva na melhora da organização das
fibras colágenas do tendão calcâneo submetido à lesão parcial.
Introduction: Calcaneous tendon is one of the most frequently damaged tendons, and its healing may last from weeks to months to be completed. In the search after speeding tendon repair, low intensity laser therapy has showed favorable effect. Objective: To assess the effect of low intensity laser therapy on the process of tissue repair of calcaneous tendon, which underwent a partial lesion, in rats. Methods: Sixty male rats were used, randomly assigned to 5 groups containing 12 animals each one; 42 of 60 underwent lesion caused by dropping a 186 g weight from a 20 cm height. In Group 1 (standard control) animals did not suffer the lesion nor underwent laser therapy; in Group 2 (control) animals suffered the lesion but did not undergo laser therapy; in Groups 3, 4, and 5 animals suffered lesion and underwent laser therapy for 3, 5, and 7 days, respectively. Animals which suffered lesion were sacrificed on the 8 th day after the lesion and assessed by polarization microscopy to analyze the degree of collagen fibers organization. Results: Both experimental and standard control Groups presented significant values when compared with the control Groups, and there was not significant difference when Groups 1 and 4 were compared, and the same occurred between Groups 3 and 5. Conclusion: Low intensity laser therapy was effective for improving collagen fibers organization of the calcaneous tendon, after it underwent a partial lesion.
Introduction: Calcaneous tendon is one of the most frequently damaged tendons, and its healing may last from weeks to months to be completed. In the search after speeding tendon repair, low intensity laser therapy has showed favorable effect. Objective: To assess the effect of low intensity laser therapy on the process of tissue repair of calcaneous tendon, which underwent a partial lesion, in rats. Methods: Sixty male rats were used, randomly assigned to 5 groups containing 12 animals each one; 42 of 60 underwent lesion caused by dropping a 186 g weight from a 20 cm height. In Group 1 (standard control) animals did not suffer the lesion nor underwent laser therapy; in Group 2 (control) animals suffered the lesion but did not undergo laser therapy; in Groups 3, 4, and 5 animals suffered lesion and underwent laser therapy for 3, 5, and 7 days, respectively. Animals which suffered lesion were sacrificed on the 8 th day after the lesion and assessed by polarization microscopy to analyze the degree of collagen fibers organization. Results: Both experimental and standard control Groups presented significant values when compared with the control Groups, and there was not significant difference when Groups 1 and 4 were compared, and the same occurred between Groups 3 and 5. Conclusion: Low intensity laser therapy was effective for improving collagen fibers organization of the calcaneous tendon, after it underwent a partial lesion.
Descrição
Citação
OLIVEIRA, Flavia Schlittler. Terapia a laser de baixa intensidade na reparação do tendão do calcâneo de ratos, submetido a lesão parcial 2006. 87 f. Dissertação (Mestrado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2006.