Alterações degenerativas induzidas pela privação de sono paradoxal em glândula sublingual de ratos
Data
2023-04-26
Autores
Aguiar, Gabriel Carvalhal de 
Orientadores
Ribeiro, Daniel Araki
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
O ser humano passa cerca de um terço de sua vida dormindo, processo este fundamental para a manutenção e equilíbrio dos mecanismos psicossocial e biológico. O sono é um processo natural e complexo, e a falta dele pode acarretar e/ou agravar problemas de saúde, tais como diabetes, hipertensão, depressão, diminuição da função imunológica, dentre outros. Nesse contexto, o presente estudo teve como objetivo avaliar se a privação de sono é capaz de deflagrar processo proliferativo e apoptótico nas glândulas sublinguais de ratos. Para isso, 24 ratos foram distribuídos em três grupos, Controle (n=8), no qual os animais não foram submetidos a qualquer procedimento; Privação do Sono (n=8) os animais foram submetidos por um período de 96 horas consecutivas à privação de sono; e Rebote (n=8), os animais foram submetidos à privação de sono paradoxal por 96 horas consecutivas, seguidos por 96 horas sem intervenção. Foram investigadas alterações histopatológicas e imunoexpressões das proteínas Ki-67, p16, Caspase-3 clivada e BCL-2. Os resultados demonstraram que a privação de sono paradoxal induziu degeneração tecidual a partir da presença de picnose, vacúolos e áreas de retenção salivar, nos grupos experimentais. A expressão de Caspase 3 clivada e bcl2 aumentou tantos nos grupos privação de sono como rebote. A análise do ki-67 demonstrou um aumento da expressão somente no grupo rebote, associado à diminuição da proteína p16. Com isso, a privação do sono se mostra capaz de deflagrar processo degenerativo no parênquima glandular, por meio da desregulação da apoptose e atividade proliferativa na glândula sublingual de ratos.
Human beings spend about one third of their lives sleeping, a process that is fundamental for the maintenance and balance of the psychosocial and biological mechanisms. Sleep is a natural and complex process, and the lack of it can cause and/or worsen health problems such as diabetes, hypertension, depression, and decreased immune function, among others. In this context, the present study aimed to evaluate whether sleep deprivation can trigger proliferative and apoptotic processes in rat sublingual glands. For this a total of 24 male were distributed into three groups: Control (n=8), in which the animals were not subjected to any procedure; Sleep Deprivation (n=8) in which the animals were submitted to sleep deprivation for 96h; Rebound (n=8), in which the animals were subjected to paradoxical sleep deprivation for 96 consecutive hours followed by 96 hours without intervention. Morphological alterations such as acinar atrophy, hypertrophy, hyperplasia and metaplasia will be investigated, we will also evaluate the immunorexpressions of proteins such as Ki-67, p16, activate Caspase-3 and BCL-2. The results showed that paradoxical sleep deprivation induced tissue degeneration from the presence of pyknosis, vacuoles and areas of salivary retention, in the experimental groups. Expression of cleaved caspase 3 and BCL-2 increased in both sleep deprivation and rebound groups. The analysis of Ki-67 showed an increase in expression only in the rebound group, associated with a decrease in p16. Therefore, sleep deprivation can trigger a degenerative process in the tissue, through the dysregulation of apoptosis and proliferative activity in the sublingual gland of rats.
Human beings spend about one third of their lives sleeping, a process that is fundamental for the maintenance and balance of the psychosocial and biological mechanisms. Sleep is a natural and complex process, and the lack of it can cause and/or worsen health problems such as diabetes, hypertension, depression, and decreased immune function, among others. In this context, the present study aimed to evaluate whether sleep deprivation can trigger proliferative and apoptotic processes in rat sublingual glands. For this a total of 24 male were distributed into three groups: Control (n=8), in which the animals were not subjected to any procedure; Sleep Deprivation (n=8) in which the animals were submitted to sleep deprivation for 96h; Rebound (n=8), in which the animals were subjected to paradoxical sleep deprivation for 96 consecutive hours followed by 96 hours without intervention. Morphological alterations such as acinar atrophy, hypertrophy, hyperplasia and metaplasia will be investigated, we will also evaluate the immunorexpressions of proteins such as Ki-67, p16, activate Caspase-3 and BCL-2. The results showed that paradoxical sleep deprivation induced tissue degeneration from the presence of pyknosis, vacuoles and areas of salivary retention, in the experimental groups. Expression of cleaved caspase 3 and BCL-2 increased in both sleep deprivation and rebound groups. The analysis of Ki-67 showed an increase in expression only in the rebound group, associated with a decrease in p16. Therefore, sleep deprivation can trigger a degenerative process in the tissue, through the dysregulation of apoptosis and proliferative activity in the sublingual gland of rats.
Descrição
Citação
AGUIAR, Gabriel Carvalhal de. Alterações degenerativas induzidas pela privação de sono paradoxal em glândula sublingual de ratos. 2023. 49 f. Dissertação (Mestrado em Bioprodutos e Bioprocessos) - Instituto de Saúde e Sociedade, Universidade Federal de São Paulo, Santos, 2023.