Fungal diversity in deep-sea sediments associated with asphalt seeps at the Sao Paulo Plateau

Fungal diversity in deep-sea sediments associated with asphalt seeps at the Sao Paulo Plateau

Author Nagano, Yuriko Google Scholar
Miura, Toshiko Google Scholar
Nishi, Shinro Google Scholar
Lima, Andre O. Google Scholar
Nakayama, Cristina Autor UNIFESP Google Scholar
Pellizari, Vivian H. Google Scholar
Fujikura, Katsunori Google Scholar
Abstract We investigated the fungal diversity in a total of 20 deep-sea sediment samples (of which 14 samples were associated with natural asphalt seeps and 6 samples were not associated) collected from two different sites at the Sao Paulo Plateau off Brazil by Ion Torrent PGM targeting ITS region of ribosomal RNA. Our results suggest that diverse fungi (113 operational taxonomic units (OTUs) based on clustering at 97% sequence similarity assigned into 9 classes and 31 genus) are present in deep-sea sediment samples collected at the Sao Paulo Plateau, dominated by Ascomycota (74.3%), followed by Basidiomycota (11.5%), unidentified fungi (7.1%), and sequences with no affiliation to any organisms in the public database (7.1%). However, it was revealed that only three species, namely Penicillium sp., Cadophora malorum and Rhodosporidiwn diobovatum, were dominant, with the majority of OTUs remaining a minor community. Unexpectedly, there was no significant difference in major fungal community structure between the asphalt seep and non-asphalt seep sites, despite the presence of mass hydrocarbon deposits and the high amount of macro organisms surrounding the asphalt seeps. However, there were some differences in the minor fungal communities, with possible asphalt degrading fungi present specifically in the asphalt seep sites. In contrast, some differences were found between the two different sampling sites. Classification of OTUs revealed that only 47 (41.6%) fungal OTUs exhibited >97% sequence similarity, in comparison with pre-existing ITS sequences in public databases, indicating that a majority of deep-sea inhabiting fungal taxa still remain undescribed. Although our knowledge on fungi and their role in deep-sea environments is still limited and scarce, this study increases our understanding of fungal diversity and community structure in deep-sea environments.
Keywords Deep-sea
Ion torrent
Asphalt seep
Sao Paulo Plateau, Shinkai 6500, lata-piuna cruise
xmlui.dri2xhtml.METS-1.0.item-coverage Oxford
Language English
Sponsor Japan Society for the Promotion of Science
Grant number JSPS: 23770098
JSPS: 15K18601
Date 2017
Published in Deep-Sea Research Part Ii-Topical Studies In Oceanography. Oxford, v. 146, p. 59-67, 2017.
ISSN 0967-0645 (Sherpa/Romeo, impact factor)
Publisher Pergamon-Elsevier Science Ltd
Extent 59-67
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000423245400009

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