Maternal seizures can affect the brain developing of offspring
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Date
2016
Authors
Cossa, Ana Carolina [UNIFESP]
Lima, Daiana Correia [UNIFESP]
do Vale, Tiago Gurgel
Alves de Alencar Rocha, Anna Karynna [UNIFESP]
Naffah-Mazzacoratti, Maria da Graca [UNIFESP]
da Silva Fernandes, Maria Jose [UNIFESP]
Amado, Debora [UNIFESP]
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Abstract
To elucidate the impact of maternal seizures in the developing rat brain, pregnant Wistar rats were subjected to the pilocarpine-induced seizures and pups from different litters were studied at different ages. In the first 24 h of life, blood glucose and blood gases were analyzed. C-14-leucine [C-14-Leu] incorporation was used to analyze protein synthesis at PN1, and Western Blot method was used to analyze protein levels of Bax, Bcl-2 and Poly(ADP-ribose) polymerase-1 (PARP-1) in the hippocampus (PN3-PN21). During the first 22 days of postnatal life, body weight gain, length, skull measures, tooth eruption, eye opening and righting reflex have been assessed. Pups from naive mothers were used as controls. Experimental pups showed a compensated metabolic acidosis and hyperglycemia. At PN1, the [C-14-Leu] incorporation into different studied areas of experimental pups was lower than in the control pups. During development, the protein levels of Bax, Bcl-2 and PARP-1 in the hippocampus of experimental pups were altered when compared with control pups. A decreased level of pro- and anti-apoptotic proteins was verified in the early postnatal age (PN3), and an increased level of pro-apoptotic proteins concomitant with a reduced level of anti-apoptotic protein was observed at the later stages of the development (PN21). Experimental pups had a delay in postnatal growth and development beyond disturb in protein synthesis and some protein expression during development. These changes can be result from hormonal alterations linked to stress and/or hypoxic events caused by maternal epileptic seizures during pregnancy.
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Metabolic Brain Disease. New York, v. 31, n. 4, p. 891-900, 2016.