Capillary electrophoresis coupled to contactless conductivity detection for analysis of amino acids of agricultural interest in composting

Capillary electrophoresis coupled to contactless conductivity detection for analysis of amino acids of agricultural interest in composting

Author Cappelini, Luciana Teresa Dias Autor UNIFESP Google Scholar
Menegoci Eugenio, Patricia de Fatima Google Scholar
Gomes Carneiro Leao, Paulo Augusto Google Scholar
Alberice, Juliana Vieira Google Scholar
Urbaczek, Ana Carolina Google Scholar
Assuncao, Nilson Antonio Autor UNIFESP Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Carrilho, Emanuel Google Scholar
Abstract Composting is a sustainable approach to manage animal and vegetal waste generated in the Fundacao Parque Zoologico de Sao Paulo. The resulting compost is often used in ZOO's premises as an organic fertilizer for the production of vegetables, which is further used to feed the animals. The composting product provides many forms of mineral and also amino acids (AA) that are absorbed by plants as nutrients. Since most amino acids absorb only slightly or not at all in the UV wavelengths, we developed a method for the determination of AA of agricultural interest in the composting samples. Due to the complexity of samples, we used ion exchange chromatography for the purification of AA prior to analysis. The proposed CZE-(CD)-D-4 method allowed a separation of the AA in a short analysis time (less than 3.0 min), with great linearity (with R-2 ranging from 0.993 to 0.998). Using a BGE of 10 mmol/L TEA, reduction of high-frequency noise and lower baseline fluctuations were obtained. The LOQ for the five AA were around 35 mu mol/L, and were adequate for our purpose. In addition, the method showed good precision (RSD of peak area and migration time less than 1.55 and 1.16%, respectively).
Keywords Amino acids
CE-C4D
Cation exchange chromatography
Composting
xmlui.dri2xhtml.METS-1.0.item-coverage Hoboken
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2014/07037-0
CNPq: 163888/2013-2
CNPq: 482696/2011-7
CNPq: 311323/2011-1
Date 2016
Published in Electrophoresis. Hoboken, v. 37, n. 19, p. 2449-2457, 2016.
ISSN 0173-0835 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 2449-2457
Origin http://dx.doi.org/10.1002/elps.201600302
Access rights Closed access
Type Article
Web of Science ID WOS:000388595400002
URI https://repositorio.unifesp.br/handle/11600/56961

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