HMSH2 and HMSH6 gene expression profiles in colorectal adenocarcinoma in patients up to 50 years of age

HMSH2 and HMSH6 gene expression profiles in colorectal adenocarcinoma in patients up to 50 years of age

Author Germini, Demetrius Google Scholar
Gehrke, Flavia Google Scholar
Lira, Daniel Google Scholar
Alves, Beatriz Google Scholar
Azzalis, Ligia Autor UNIFESP Google Scholar
Perez, Matheus Google Scholar
Fonseca, Fernando Autor UNIFESP Google Scholar
Waisberg, Jaques Google Scholar
Abstract Lynch syndrome, previously called hereditary non-polyposis colorectal cancer (HNPCC), is a major mortality threat. It is an autosomal dominant disease which is caused by a germline mutation in the DNA mismatch repair (MMR), especially in patients aged up to 50 years. Such mutation more frequently occurs in the hMSH2 gene (38-40%) and less frequently in the hMSH6 gene (14-16%). These mutations, when associated with the patient's lifestyle, may reveal a considerable variability in the disease manifestations, such as the degrees of penetrance and clinical aggressiveness. The aim of this study is to analyze the expression of DNA MMR genes, and correlate this expression with the clinical and anatomopathological findings of the neoplasia in patients aged between 39 and 49 years. A total of 45 patients were included: (48.9%) males and (51.1%) females, and they all underwent resection of a colorectal adenocarcinoma. The tissue microarray technique was used to analyze the relative and absolute expression of hMSH2 and hMSH6. Amsterdam II criteria for the diagnosis of HNPCC were obtained from the data provided by medical records and interviews with patients. hMSH2 and hMSH6 was expressed in all patients, which correlated between each other (RHO = 0.669 and p < 0.001) but not to age. There is a positive correlation between the expressions of males (RHO = 0.673 and p = 0.001) and females (RHO = 0.006 and p < 0.001). It is possible to evaluate the expression of MMR genes in embedded anatomopathological samples. Gene expressions correlated between each other and to the sex of the patients, but no difference in relation to age. (C) 2016 Elsevier Masson SAS. All rights reserved.
Keywords Lynch syndrome
DNA mismatch repair
hMSH2
hMSH6: hereditary non-polyposis colorectal cancer
Colorectal adenocarcinoma
xmlui.dri2xhtml.METS-1.0.item-coverage Paris
Language English
Date 2016
Published in Biomedicine & Pharmacotherapy. Paris, v. 83, p. 602-606, 2016.
ISSN 0753-3322 (Sherpa/Romeo, impact factor)
Publisher Elsevier France-Editions Scientifiques Medicales Elsevier
Extent 602-606
Origin http://dx.doi.org/10.1016/j.biopha.2016.07.015
Access rights Closed access
Type Article
Web of Science ID WOS:000390433400072
URI https://repositorio.unifesp.br/handle/11600/56949

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