Synthesis and Characterization of Methylene Blue-Containing Silica-Coated Magnetic Nanoparticles for Photodynamic Therapy
Fudimura, Karina Amancio [UNIFESP]
Seabra, Amedea Barozzi [UNIFESP]
Santos, Marconi da Cruz [UNIFESP]
Haddad, Paula Silvia [UNIFESP]
Is part ofJournal Of Nanoscience And Nanotechnology
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Superparamagnetic iron oxide nanoparticles (SPIONs), with appropriate surface coating, are commonly used for biomedical applications such as photodynamic therapy (PDT). This work describes the preparation and characterization of methylene blue (MB)-containing silica-coated SPIONs. Upon exposure to light, MB reacts with molecular oxygen and generates singlet oxygen (O-1(2)) which is cytotoxic and causes irreversible damage to tumor tissues. In this work, SPIONs were synthesized by co-precipitation and coated with a single/double silica layer. The photoactive molecule MB was entrapped in the silica layer deposited on the surface of SPIONs, leading to the formation of hybrid nanomaterials composed of a magnetic core and silica layer. The nanocomposite exhibited magnetic behavior at room temperature due to the presence of its Fe3O4 core. Structural and morphological characterizations were performed by X-ray diffraction (XRD), Fourier transformed infrared (FTIR), SQUID magnetic measurements, ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and dynamic light scattering. The results showed the presence of a crystalline Fe3O4 magnetic core and amorphous silica phases. Kinetic measurements revealed O-1(2) generation by the nanoparticles upon irradiation with visible light (lambda = 532 nm or lambda = 633 nm). The results highlight the potential uses of SPIONs coated with MB-entrapped silica for PDT, whereby a sustained and localized generation of O-1(2) was successfully achieved.
CitationJournal Of Nanoscience And Nanotechnology. Valencia, v. 17, n. 1, p. 133-142, 2017.
KeywordsIron Oxide Nanoparticles
SponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Brazilian Network on Nanotoxicology (MCTI/CNPq)
Laboratory of Nanostructure Synthesis and Biosystem Interactions-NANOBIOSS (MCTI)
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