Design and characterization of crotamine-functionalized gold nanoparticles

Design and characterization of crotamine-functionalized gold nanoparticles

Author Karpel, Richard L. Google Scholar
Liberato, Michelle da Silva Google Scholar
Campeiro, Joana Darc Autor UNIFESP Google Scholar
Bergeon, Lorna Google Scholar
Szychowski, Brian Google Scholar
Butler, Andrew Google Scholar
Marino, Giovanni Google Scholar
Cusic, Joelle F. Google Scholar
Oliveira, Lilian Caroline Goncalves de Autor UNIFESP Google Scholar
Oliveira, Eduardo B. Google Scholar
Farias, Marcelo Alexandre de Google Scholar
Portugal, Rodrigo Villares Google Scholar
Alves, Wendel Andrade Google Scholar
Daniel, Marie-Christine Google Scholar
Hayashi, Mirian Akemi Furuie Autor UNIFESP Google Scholar
Abstract This paper describes the development of a facile and environmentally friendly strategy for supporting crotamine on gold nanoparticles (GNPs). Our approach was based on the covalent binding interaction between the cell penetrating peptide crotamine, which is a snake venom polypeptide with preference to penetrate dividing cells, and a polyethylene glycol (PEG) ligand, which is a nontoxic, water-soluble and easily obtainable commercial polymer. Crotamine was derivatized with ortho-pyridyldisulfide-polyethyleneglycol-N-hydroxysuccinimide (OPSS-PEG-SVA) cross-linker to produce OPSS-PEG-crotamine as the surface modifier of GNP. OPSS-PEG-SVA can serve not only as a surface modifier, but also as a stabilizing agent for GNPs. The successful PEGylation of the nanoparticles was demonstrated using different physicochemical techniques, while the grafting densities of the PEG ligands and crotamine on the surface of the nanoparticles were estimated using a combination of electron microscopy and mass spectrometry analysis. In vitro assays confirmed the internalization of these GNPs, into living HeLa cells. The results described herein suggest that our approach may serve as a simple platform for the synthesis Of GNPs decorated with crotamine with well-defined morphologies and uniform dispersion, opening new roads for crotamine biomedical applications. (C) 2017 Elsevier B.V. All rights reserved.
Keywords Snake toxin
Crotamine
Gold nanoparticle
Polyethylene glycol
Polypeptide
Cell penetrating peptide
xmlui.dri2xhtml.METS-1.0.item-coverage Amsterdam
Language English
Sponsor Sao Paulo Research Foundation (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo FAPESP)
National Council of Technological and Scientific Development (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico CNPq)
National Science Foundationx
UMBC Designated Resarch Initiative Fund
Brazil Scientific Mobility Program
Undergraduate Research Award from UMBC
National Institutes of Health SIG grant
Grant number FAPESP: 2013/13392-4
FAPESP: 2015/24018-1
FAPESP: 2017/02413-1
CNPq: 477760/2010-4
CNPq: 557753/2010-4
CNPq: 508113/2010-5
CNPq: 311815/2012-0
CNPq: 475739/2013-2
CNPq: 302923/2015-2
CNPq: 309337/2016-0
NSF: CHE 1507462
NIH: 1S10RR26870-1
Date 2018
Published in Colloids And Surfaces B-Biointerfaces. Amsterdam, v. 163, p. 43313, 2018.
ISSN 0927-7765 (Sherpa/Romeo, impact factor)
Publisher Elsevier Science Bv
Extent 1-8
Origin http://dx.doi.org/10.1016/j.colsurfb.2017.12.013
Access rights Closed access
Type Article
Web of Science ID WOS:000427217300001
URI https://repositorio.unifesp.br/handle/11600/55918

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