Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil

Rotavirus genotypes as etiological agents of diarrhoea in general populations of two geographic regions of Brazil

Author Leite, Marcel Autor UNIFESP Google Scholar
Compagnoli Carmona, Rita de Cassia Google Scholar
Carraro, Emerson Google Scholar
Aranha Watanabe, Aripuana Sakurada Autor UNIFESP Google Scholar
Hernandes Granato, Celso Francisco Autor UNIFESP Google Scholar
Abstract Rotavirus is the main global cause of severe childhood diarrhoea among children. In 2006, Rotarix r (G1P[8]) was introduced into Brazil's National Immunization Program. The vaccine coverage rate was 84.4% in 2009. Evidences of increasing G2P[4] after 2006 opened up the discussion about the vaccine effectiveness to non-G1 strains. The aim of this study was to identify the circulating rotavirus genotypes in two Brazilian regions during 2009. A total of 223 positive samples by immunochromatography and latex agglutination assay from the Northeast (Bahia/Pernambuco States) and Southeast (Sao Paulo/Rio de Janeiro States) regions were included in the study. The samples were submitted to genotyping by nested-PCR according to VP7(G) and VP4(P) and 175 samples (78.5%) were able to be characterized. Considering the characterization of VP7, the G-types detected were G1, G2, and G4 in the Northeast, and G2, G3, G5, and G9 in the Southeast. Considering the characterization of VP4, the P-types detected were P[4], P[8], and P[6]/P[9] in the Northeast and the Southeast. The most frequent mixed types found were G2P[4]/G2P[NT](81.4%), G2P[6](5.2%), G1P[6](5.2%) in the Northeast, and G2P[4]/G2P[NT](78.8%), G2P[6](8.2%), G9P[8](4.7%) in the Southeast. Among immunized individuals whose age ranged from 0- 4 years, the G2P[4]/G2P[NT] genotype was identified in 91,0% of cases, and among non-immunized individuals of the same age, the G2P[4]/G2P[NT] genotype was identified in 85.7% of the cases. In accordance with the high level of vaccine coverage, the data suggest that the circulation of G2P[4] in these regions had a considerable increase after the introduction of Rotarix (R).
Keywords Rotavirus
Epidemiology
Genotype
Vaccine
xmlui.dri2xhtml.METS-1.0.item-coverage Sao Paulo
Language English
Sponsor Federal University of Sao Paulo
Fleury Group
Date 2017
Published in Revista Do Instituto De Medicina Tropical De Sao Paulo. Sao Paulo, v. 59, p. -, 2017.
ISSN 0036-4665 (Sherpa/Romeo, impact factor)
Publisher Inst Medicina Tropical Sao Paulo
Extent -
Origin http://dx.doi.org/10.1590/S1678-9946201759045
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000407427600002
SciELO ID S0036-46652017005000223 (statistics in SciELO)
URI https://repositorio.unifesp.br/handle/11600/55497

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