Lead (Pb) exposure induces disturbances in epigenetic status in workers exposed to this metal
Devoz, Paula Picoli
Gomes, Willian Robert
Araújo, Marília Ladeira [UNIFESP]
Ribeiro, Diego Luis
Antunes, Lusânia Maria Greggi
Batista, Bruno Lemos
Barbosa, Fernando, Jr.
Barcelos, Gustavo Rafael Mazzaron [UNIFESP]
Is part ofJournal Of Toxicology And Environmental Health-Part A-Current Issues
International Meeting of Environmental Health and Toxicology (IMEHTOX) - Advances in Toxicology and Environmental Health in Brazil
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Previous studies showed that lead (Pb) exposure may modulate gene expression by changes in the epigenetic status. However, little is known about the impact of Pb exposure and alterations on DNA methylation patterns in humans exposed to this metal. The aim of this study was to assess the consequences of exposure to Pb on DNA global methylation, in order to gain a better understanding of the interactions between Pb exposure and epigenetic effects. The study included 100 male workers employed in automotive battery factories from Paraná State, Brazil. Concentrations of Pb in blood (B-Pb) and plasma (P-Pb) were determined by ICP-MS, the percentage (%) of global DNA methylation was determined by quantification of 5-methylcytosine using indirect ELISA, and sociodemographic data collected by questionnaire by trained interviewers. The mean age was 37 ± 10 (18–67 years); 18% of participants were smokers, while 32% reported consumption of alcoholic beverages. The B-Pb and P-Pb levels were 20 ± 11 and 0.56 ± 0.64 µg/dl, respectively; % global DNA methylation was 2.8 ± 1.1% (ranging from 1.1 to 6.5%). B-Pb and P-Pb concentrations were significantly correlated. Furthermore, a marked association was noted between Pb biomarkers and DNA global methylation. Taken together, our data demonstrated that Pb exposure induced alterations on DNA global methylation in workers who were exposed to the metal and consequently may result in disturbances in the regulation of gene expression, leading to potentially several health adverse effect outcomes.
CitationJournal Of Toxicology And Environmental Health-Part A-Current Issues. Philadelphia, v. 80, n. 19-21, p. 1098-1105, 2017.
SponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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