F-19 labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy
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2017
Autores
Lima, Marcelo A. [UNIFESP]
Cavalheiro, Renan P. [UNIFESP]
Viana, Gustavo M. [UNIFESP]
Meneghetti, Maria C. Z. [UNIFESP]
Rudd, Timothy R.
Skidmore, Mark A.
Powell, Andrew K.
Yates, Edwin A. [UNIFESP]
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Studying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enable a proper comparison of results over these different scales but, often, this is not feasible. The cell surface and extracellular matrix polysaccharides, glycosaminoglycans (GAGs) lack groups that can be detected selectively in the biological milieu. The introduction of F-19 labels into GAG polysaccharides is explored and the interaction of a labelled GAG with the heparin-binding protein, antithrombin, employing F-19 NMR spectroscopy is followed. Furthermore, the ability of F-19 labelled GAGs to be imaged using CARS microscopy is demonstrated. F-19 labelled GAGs enable both F-19 NMR protein-GAG binding studies in solution at the molecular level and non-linear microscopy at a microscopic scale to be conducted on the same material, essentially free of background signals.
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Glycoconjugate Journal. Dordrecht, v. 34, n. 3, p. 405-410, 2017.