F-19 labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy

Página do item simplificado
dc.citation.issue3
dc.citation.volume34
dc.contributor.authorLima, Marcelo A. [UNIFESP]
dc.contributor.authorCavalheiro, Renan P. [UNIFESP]
dc.contributor.authorViana, Gustavo M. [UNIFESP]
dc.contributor.authorMeneghetti, Maria C. Z. [UNIFESP]
dc.contributor.authorRudd, Timothy R.
dc.contributor.authorSkidmore, Mark A.
dc.contributor.authorPowell, Andrew K.
dc.contributor.authorYates, Edwin A. [UNIFESP]
dc.coverageDordrecht
dc.date.accessioned2020-07-13T11:53:01Z
dc.date.available2020-07-13T11:53:01Z
dc.date.issued2017
dc.description.abstractStudying polysaccharide-protein interactions under physiological conditions by conventional techniques is challenging. Ideally, macromolecules could be followed by both in vitro spectroscopy experiments as well as in tissues using microscopy, to enable a proper comparison of results over these different scales but, often, this is not feasible. The cell surface and extracellular matrix polysaccharides, glycosaminoglycans (GAGs) lack groups that can be detected selectively in the biological milieu. The introduction of F-19 labels into GAG polysaccharides is explored and the interaction of a labelled GAG with the heparin-binding protein, antithrombin, employing F-19 NMR spectroscopy is followed. Furthermore, the ability of F-19 labelled GAGs to be imaged using CARS microscopy is demonstrated. F-19 labelled GAGs enable both F-19 NMR protein-GAG binding studies in solution at the molecular level and non-linear microscopy at a microscopic scale to be conducted on the same material, essentially free of background signals.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Biochem, Rua Tres Maio, BR-40440 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Liverpool, Dept Biochem, Liverpool L69 7ZB, Merseyside, England
dc.description.affiliationNatl Inst Biol Stand & Controls, Blanche Lane, Potters Bar EN6 3QG, Herts, England
dc.description.affiliationKeele Univ, Sch Life Sci, Keele ST5 5BG, Staffs, England
dc.description.affiliationLiverpool John Moores Univ, Sch Pharm & Biomol Sci, Liverpool L3 3AF, Merseyside, England
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biochem, Rua Tres Maio, BR-40440 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.format.extent405-410
dc.identifierhttp://dx.doi.org/10.1007/s10719-016-9723-x
dc.identifier.citationGlycoconjugate Journal. Dordrecht, v. 34, n. 3, p. 405-410, 2017.
dc.identifier.doi10.1007/s10719-016-9723-x
dc.identifier.issn0282-0080
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54358
dc.identifier.wosWOS:000403322800012
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofGlycoconjugate Journal
dc.rightsAcesso restrito
dc.subjectHeparinen
dc.subjectNMRen
dc.subjectF-19en
dc.subjectNon-linear microscopyen
dc.titleF-19 labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopyen
dc.typeArtigo
Arquivos
Coleções
EPM - Artigos