Overexpression of -synuclein in an astrocyte cell line promotes autophagy inhibition and apoptosis
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2018
Autores
Erustes, Adolfo Garcia [UNIFESP]
Stefani, Fernanda Yakel [UNIFESP]
Terashima, Juliana Yoshie [UNIFESP]
Stilhano, Roberta Sessa [UNIFESP]
Monteforte, Priscila Totarelli [UNIFESP]
da Silva Pereira, Gustavo Jose [UNIFESP]
Han, Sang Won [UNIFESP]
Calgarotto, Andrana Karla
Hsu, Yi-Te
Ureshino, Rodrigo Portes [UNIFESP]
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α‐Synuclein is the major component of neuronal cytoplasmic aggregates called Lewy bodies, the main pathological hallmark of Parkinson disease. Although neurons are the predominant cells expressing α‐synuclein in the brain, recent studies have demonstrated that primary astrocytes in culture also express α‐synuclein and regulate α‐synuclein trafficking. Astrocytes have a neuroprotective role in several detrimental brain conditions; we therefore analyzed the effects of the overexpression of wild‐type α‐synuclein and its A30P and A53T mutants on autophagy and apoptosis. We observed that in immortalized astrocyte cell lines, overexpression of α‐synuclein proteins promotes the decrease of LC3‐II and the increase of p62 protein levels, suggesting the inhibition of autophagy. When these cells were treated with rotenone, there was a loss of mitochondrial membrane potential, especially in cells expressing mutant α‐synuclein. The level of this decrease was related to the toxicity of the mutants because they show a more intense and sustained effect. The decrease in autophagy and the mitochondrial changes in conjunction with parkin expression levels may sensitize astrocytes to apoptosis.
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Journal Of Neuroscience Research. Hoboken, v. 96, n. 1, p. 160-171, 2018.