Establishment and Partial Characterization of an Epirubicin-Resistant Gastric Cancer Cell Line with Upregulated ABCB1
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2014-01-01
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Felipe, Aledson Vitor [UNIFESP]
Moraes, Andrea Aparecida Fátima Souza [UNIFESP]
Oliveira, Juliana de [UNIFESP]
Silva, Tiago Donizetti da [UNIFESP]
Forones, Nora Manoukian [UNIFESP]
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Multidrug resistance (MDR) is a major impediment to successful chemotherapy of gastric cancer. Our aim was to establish an epirubicin-resistant cell subline (AGS/EPI) and to elucidate the mechanisms involved in acquired EPI resistance. the AGS/EPI cell subline developed by exposing parental AGS cells to stepwise increasing concentrations of EPI demonstrated 2.52-fold resistance relative to the AGS cell line, and mRNA expression of the ATP-dependent drug-efflux pump P-glycoprotein (Pgp), more recently known as ABCB1 protein, was similarly upregulated. An AGS/EPI cell subline could thus be effectively established, and MDR mechanism of these cells was shown to be related to the overexpression of mRNA of the ABCB1 gene.
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Asian Pacific Journal of Cancer Prevention. Gyeonggi-do: Asian Pacific Organization Cancer Prevention, v. 15, n. 16, p. 6849-6853, 2014.