Chronic unpredictable mild stress alters an anxiety-related defensive response, Fos immunoreactivity and hippocampal adult neurogenesis

Chronic unpredictable mild stress alters an anxiety-related defensive response, Fos immunoreactivity and hippocampal adult neurogenesis

Author Andrade, José Simões de Autor UNIFESP Google Scholar
Céspedes, Isabel Cristina Autor UNIFESP Google Scholar
Abrão, Renata Oliveira Autor UNIFESP Google Scholar
Santos, Thays Brenner dos Autor UNIFESP Google Scholar
Diniz, Leila Autor UNIFESP Google Scholar
Britto, Luiz Roberto Giorgetti de Google Scholar
Spadari-Bratfisch, Regina Celia Autor UNIFESP Google Scholar
Ortolani, Daniela Autor UNIFESP Google Scholar
Melo-Thomas, Liana Autor UNIFESP Google Scholar
Silva, Regina Cláudia Barbosa da Autor UNIFESP Google Scholar
Viana, Milena de Barros Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Previous results show that elevated T-maze (ETM) avoidance responses are facilitated by acute restraint. Escape, on the other hand, was unaltered. To examine if the magnitude of the stressor is an important factor influencing these results, we investigated the effects of unpredictable chronic mild stress (UCMS) on ETM avoidance and escape measurements. Analysis of Fos protein immunoreactivity (Fos-ir) was used to map areas activated by stress exposure in response to ETM avoidance and escape performance. Additionally, the effects of the UCMS protocol on the number of cells expressing the marker of migrating neuroblasts doublecortin (DCX) in the hippocampus were investigated. Corticosterone serum levels were also measured. Results showed that UCMS facilitates ETM avoidance, not altering escape. in unstressed animals, avoidance performance increases Fos-ir in the cingulate cortex, hippocampus (dentate gyrus) and basomedial amygdala, and escape increases Fos-ir in the dorsolateral periaqueductal gray and locus ceruleus. in stressed animals submitted to ETM avoidance, increases in Fos-ir were observed in the cingulate cortex, ventrolateral septum, hippocampus, hypothalamus, amygdala, dorsal and median raphe nuclei. in stressed animals submitted to ETM escape, increases in Fos-ir were observed in the cingulate cortex, periaqueductal gray and locus ceruleus. Also, UCMS exposure decreased the number of DCX-positive cells in the dorsal and ventral hippocampus and increased corticosterone serum levels. These data suggest that the anxiogenic effects of UCMS are related to the activation of specific neurobiological circuits that modulate anxiety and confirm that this stress protocol activates the hypothalamus-pituitary-adrenal axis and decreases hippocampal adult neurogenesis. (C) 2013 Elsevier B.V. All rights reserved.
Keywords Unpredictable chronic mild stress
Elevated T-maze
Fos immunoreactivity
Neurogenesis
Corticosterone
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2011/17471-0
FAPESP: 2011/01409-4
Date 2013-08-01
Published in Behavioural Brain Research. Amsterdam: Elsevier B.V., v. 250, p. 81-90, 2013.
ISSN 0166-4328 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 81-90
Origin https://dx.doi.org/10.1016/j.bbr.2013.04.031
Access rights Closed access
Type Article
Web of Science ID WOS:000321880400012
URI https://repositorio.unifesp.br/handle/11600/36595

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