Acute restraint differently alters defensive responses and fos immunoreactivity in the rat brain

Acute restraint differently alters defensive responses and fos immunoreactivity in the rat brain

Author Andrade, José Simões de Autor UNIFESP Google Scholar
Abrão, Renata Oliveira Autor UNIFESP Google Scholar
Céspedes, Isabel Cristina Autor UNIFESP Google Scholar
Garcia, Marcia Carvalho Autor UNIFESP Google Scholar
Nascimento, Juliana Olivetti Guzman Autor UNIFESP Google Scholar
Spadari-Bratfisch, Regina Celia Autor UNIFESP Google Scholar
Melo-Thomas, Liana Autor UNIFESP Google Scholar
Silva, Regina Cláudia Barbosa da Autor UNIFESP Google Scholar
Viana, Milena de Barros Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Results from a previous study show that rats exposed to acute restraint display anxiogenic-like behavior, evidenced by facilitation of avoidance responses in the elevated T-maze (ETM) model of anxiety. in contrast, escape responses were unaltered by stress exposure. Since ETM avoidance and escape tasks seem to activate distinct sets of brain structures, it is possible that the differences observed with acute restraint are due to particularities in the neurobiological mechanisms which modulate these responses. in the present study, analysis of fos protein immunoreactivity (fos-ir) was used to map areas activated by exposure of male Wistar rats to restraint stress (30 min) previously (30 min) to the ETM. Corticosterone levels were also measured in stressed and non-stressed animals. Confirming previous observations restraint facilitated avoidance performance, an anxiogenic result, while leaving escape unaltered. Performance of the avoidance task increased fos-ir in the frontal cortex, intermediate lateral septum, basolateral amygdala, basomedial amygdala, lateral amygdala, anterior hypothalamus and dorsal raphe nucleus. in contrast, performance of escape increased fos-ir in the ventromedial hypothalamus, dorsolateral periaqueductal gray and locus ceruleus. Both behavioral tasks also increased fos-ir in the dorsomedial hypothalamus. Restraint significantly raised corticosterone levels. Additionally after restraint, fos-ir was predominantly seen in the basolateral amygdala and dorsal raphe of animals submitted to the avoidance task. This data confirms that different sets of brain structures are activated by ETM avoidance and escape tasks and suggests that acute restraint differently alters ETM behavior and the pattern of fos activation in the brain. (C) 2012 Elsevier B.V. All rights reserved.
Keywords Stress
Restraint
Anxiety
Panic
Corticosterone
fos immunoreactivity
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 2011/17471-0
Date 2012-06-15
Published in Behavioural Brain Research. Amsterdam: Elsevier B.V., v. 232, n. 1, p. 20-29, 2012.
ISSN 0166-4328 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 20-29
Origin https://dx.doi.org/10.1016/j.bbr.2012.03.034
Access rights Closed access
Type Article
Web of Science ID WOS:000305595600004
URI https://repositorio.unifesp.br/handle/11600/34996

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