Alterations in Calcium Signaling and a Decrease in Bcl-2 Expression: Possible Correlation With Apoptosis in Aged Striatum
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2010-02-01
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Ureshino, Rodrigo Portes [UNIFESP]
Bertoncini, Clelia Rejane Antonio [UNIFESP]
Fernandes, Maria Jose da Silva [UNIFESP]
Abdalla, Fernando Mauricio Francis
Porto, Catarina Segreti [UNIFESP]
Hsu, Yi-Te
Lopes, Guiomar Silva [UNIFESP]
Smaili, Soraya Soubhi [UNIFESP]
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Aging is a multifaceted process associated with various functional and structural deficits that might be evolved in degenerative diseases. It has been shown that neurodegenerative disorders are associated with alterations in Ca(2+) homeostasis. Thus, in the present work, we have investigated Ca(2+) signaling and apoptosis in aged striatum. Our results show that glutamate and NMDA evoke a greater Ca(2+) rise in striatum slices from aged animals. However, this difference is not present when glutamate is tested in the absence of external Ca(2+). Immunostaining of glutamate receptors shows that only NMDA receptors (NRI) are increased in the striatum of aged rats. Increases in mitochondrial Ca(2+) content and in the reactive oxygen species levels were also observed in aged animals, which could be associated with tissue vulnerability. in addition, a decrease in the Bcl-2 protein expression and an enhancement in apoptosis were also present in aged striatum. Together the results indicate that, in aged animals, alterations in Ca(2+) handling coupled to an increase in ROS accumulation and a decrease in the prosurvival protein Bcl-2 may contribute to apoptosis induction and cell death in rat striatum. (C) 2009 Wiley-Liss, Inc.
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Journal of Neuroscience Research. Hoboken: Wiley-liss, v. 88, n. 2, p. 438-447, 2010.