Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a pro-inflammatory effect in adipose tissue in rats

Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a pro-inflammatory effect in adipose tissue in rats

Author Rosa Neto, José Cesar Autor UNIFESP Google Scholar
Lira, Fabio Santos de Autor UNIFESP Google Scholar
Oyama, Lila Missae Autor UNIFESP Google Scholar
Zanchi, Nelo Eidy Google Scholar
Yamashita, Alex Shimura Google Scholar
Batista, Miguel Luiz Google Scholar
Nascimento, Claudia Maria da Penha Oller do Autor UNIFESP Google Scholar
Seelaender, Marilia Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract It is well known that exhaustive exercise increases serum and skeletal muscle IL-6 concentrations. However, the effect of exhaustive exercise on the concentrations of other cytokines in the muscle and in the adipose tissue is controversial. the purpose of this study was to evaluate the effect of exhaustive exercise on mRNA and protein expression of IL-10, TNF-alpha and IL-6 in different types of skeletal muscle (EDL, soleus) and in two different depots of white adipose tissue (mesenteric-MEAT and retroperitoneal-RPAT). Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6) and 6 (E6 group, n = 6) hours after the exhaustion protocol, which consisted of running on a treadmill (approximately 70% VO2max for 50 min and then subsequently at an elevated rate that increased at 1 m/min every minute, until exhaustion). the control group (C group, n = 6) was not subjected to exercise. Cytokine protein expression increased in EDL, soleus, MEAT and RPAT from all exercised groups, as detected by ELISA. EDL IL-10 and TNF-alpha expression was higher than that of the soleus. the IL-10/TNF-alpha ratio was increased in the skeletal muscle, especially in EDL, but it was found to be decreased in the adipose tissue. These results show that exhaustive exercise presents a different effect depending on the tissue which is analysed: in the muscle, it induces an anti-inflammatory effect, especially in type 2 fibres, while the pro-inflammatory effect prevails in adipose tissue, possibly contributing to increased lipolysis to provide energy for the exercising muscle.
Keywords Cytokines
Inflammation
Adipose tissue
Exercise
Skeletal muscle
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 04/11588-0
FAPESP: 05/01445-0
Date 2009-07-01
Published in European Journal of Applied Physiology. New York: Springer, v. 106, n. 5, p. 697-704, 2009.
ISSN 1439-6319 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 697-704
Origin https://dx.doi.org/10.1007/s00421-009-1070-1
Access rights Closed access
Type Article
Web of Science ID WOS:000267661700007
URI https://repositorio.unifesp.br/handle/11600/31631

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