Lack of effect of prior treatment with fluoride on genotoxicity of two chemical agents in vitro

Lack of effect of prior treatment with fluoride on genotoxicity of two chemical agents in vitro

Author Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Marques, Mariangela Esther Alencar Google Scholar
Salvadori, Daisy Maria Favero Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The goal of this study was to investigate the ability of fluoride to modulate the genotoxic effects induced by the oxidative agent hydrogen peroxide (H2O2) and the alkylating agent methyl methanesulfonate (MMS) in vitro by the single-cell gel ( comet) assay. Chinese hamster ovary cells were exposed in culture for 1 h at 37 degrees C to sodium fluoride at 7-100 mu g/ml. NaF-treated and control cells were then incubated with 0-10 mu M MMS in phosphate-buffered saline (PBS) for 15 min at 37 degrees C, or 7-100 mu M H2O2 in distilled water for 5 min on ice. Negative control cells were treated with PBS for 1 h at 37 degrees C. Clear concentration-related effects were observed for the two genotoxins. Increase of DNA damage induced by either MMS or H2O2 was not significantly altered by pretreatment with NaF. the data indicate that NaF does not modulate alkylation-induced genotoxicity or oxidative DNA damage as measured by the single-cell gel ( comet) assay. Copyright (c) 2007 S. Karger AG, Basel
Keywords Genotoxicity
Single cell gel (comet) assay
Sodium fluoride
Language English
Date 2007-01-01
Published in Caries Research. Basel: Karger, v. 41, n. 3, p. 239-243, 2007.
ISSN 0008-6568 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent 239-243
Origin https://dx.doi.org/10.1159/000099326
Access rights Closed access
Type Article
Web of Science ID WOS:000245758200014
URI https://repositorio.unifesp.br/handle/11600/29437

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