Melanocyte transformation associated with substrate adhesion impediment

Melanocyte transformation associated with substrate adhesion impediment

Author Oba-Shinjo, Sueli Mieko Autor UNIFESP Google Scholar
Correa, Mariangela Autor UNIFESP Google Scholar
Ricca, Tatiana Iervolino Autor UNIFESP Google Scholar
Molognoni, Fernanda Autor UNIFESP Google Scholar
Pinhal, Maria Aparecida da Silva Autor UNIFESP Google Scholar
Neves, Izabel A. Autor UNIFESP Google Scholar
Marie, Sueli Kazue Nagahashi Google Scholar
Sampaio, Lucia de Oliveira Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Chammas, Roger Google Scholar
Jasiulionis, Miriam Galvonas Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Exclude experimental models of malignant transformationemploy chemical and physical carcinogens or genetic manipulations to study tumor progression. in this work, different melanoma cell lines were established after submitting a nontumorigenic melanocyte lineage (melan-a) to sequential cycles of forced anchorage impediment. the great majority of these cells underwent anoikis when maintained in suspension. After one deadhesion cycle, phenotypic alterations were noticeable in the few surviving cells, which became more numerous and showed progressive alterations after each adhesion impediment step. No significant differences in cell surface expression of integrins were detected, but a clear electrophoretic migration shift, compatible with an altered glycosylation pattern, was observed for beta(1) chain in transformed cell lines. in parallel, a progressive enrichment of tri- and tetra-antennary N-glycans was apparent, suggesting increased N-acetylglucosaminyl-transferase V activity. Alterations both in proteoglycan glycosylation pattern and core protein expression were detected during the transformation process. in conclusion, this model corroborates the role of adhesion state as a promoting agent in transformation process and demonstrates that cell adhesion disturbances may act as carcinogenic stimuli, at least for a nontumorigenic immortalized melanocyte lineage. These findings have intriguing implications for in vivo carcinogenesis, suggesting that anchorage independence may precede, and contribute to, neoplastic conversion.
Keywords melanocyte transformation
substrate adhesion impediment
adhesion molecules
Language English
Date 2006-03-01
Published in Neoplasia. Ann Arbor: Neoplasia Press, v. 8, n. 3, p. 231-241, 2006.
ISSN 1522-8002 (Sherpa/Romeo, impact factor)
Publisher Neoplasia Press
Extent 231-241
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000239282800009

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