Melanocyte transformation associated with substrate adhesion impediment

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dc.contributor.authorOba-Shinjo, Sueli Mieko [UNIFESP]
dc.contributor.authorCorrea, Mariangela [UNIFESP]
dc.contributor.authorRicca, Tatiana Iervolino [UNIFESP]
dc.contributor.authorMolognoni, Fernanda [UNIFESP]
dc.contributor.authorPinhal, Maria Aparecida da Silva [UNIFESP]
dc.contributor.authorNeves, Izabel A. [UNIFESP]
dc.contributor.authorMarie, Sueli Kazue Nagahashi
dc.contributor.authorSampaio, Lucia de Oliveira [UNIFESP]
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorChammas, Roger
dc.contributor.authorJasiulionis, Miriam Galvonas [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T12:41:00Z
dc.date.available2016-01-24T12:41:00Z
dc.date.issued2006-03-01
dc.description.abstractExclude experimental models of malignant transformationemploy chemical and physical carcinogens or genetic manipulations to study tumor progression. in this work, different melanoma cell lines were established after submitting a nontumorigenic melanocyte lineage (melan-a) to sequential cycles of forced anchorage impediment. the great majority of these cells underwent anoikis when maintained in suspension. After one deadhesion cycle, phenotypic alterations were noticeable in the few surviving cells, which became more numerous and showed progressive alterations after each adhesion impediment step. No significant differences in cell surface expression of integrins were detected, but a clear electrophoretic migration shift, compatible with an altered glycosylation pattern, was observed for beta(1) chain in transformed cell lines. in parallel, a progressive enrichment of tri- and tetra-antennary N-glycans was apparent, suggesting increased N-acetylglucosaminyl-transferase V activity. Alterations both in proteoglycan glycosylation pattern and core protein expression were detected during the transformation process. in conclusion, this model corroborates the role of adhesion state as a promoting agent in transformation process and demonstrates that cell adhesion disturbances may act as carcinogenic stimuli, at least for a nontumorigenic immortalized melanocyte lineage. These findings have intriguing implications for in vivo carcinogenesis, suggesting that anchorage independence may precede, and contribute to, neoplastic conversion.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Micro Imuno & Parastiol, Disciplina Imunol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Dept Neurol, Mol Biol Lab, BR-05508 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Fac Med, Expt Oncol Lab, BR-05508 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Micro Imuno & Parastiol, Disciplina Imunol, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Bioquim, Disciplina Biol Mol, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent231-241
dc.identifierhttp://dx.doi.org/10.1593/neo.05781
dc.identifier.citationNeoplasia. Ann Arbor: Neoplasia Press, v. 8, n. 3, p. 231-241, 2006.
dc.identifier.doi10.1593/neo.05781
dc.identifier.issn1522-8002
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28761
dc.identifier.wosWOS:000239282800009
dc.language.isoeng
dc.publisherNeoplasia Press
dc.relation.ispartofNeoplasia
dc.rightsAcesso aberto
dc.subjectmelanocyte transformationen
dc.subjectsubstrate adhesion impedimenten
dc.subjectadhesion moleculesen
dc.subjectN-glycansen
dc.subjectproteoglycansen
dc.titleMelanocyte transformation associated with substrate adhesion impedimenten
dc.typeArtigo
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