Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method
Nenhuma Miniatura disponível
Data
2004-05-01
Autores
Endo, S.
Komori, T.
Ricci, Giannina [UNIFESP]
Sano, A.
Yokoyama, K.
Ohori, A.
Kamei, K.
Franco, Marcello Fabiano de [UNIFESP]
Miyaji, M.
Nishimura, K.
Orientadores
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Descrição
Citação
Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.