Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method

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dc.contributor.authorEndo, S.
dc.contributor.authorKomori, T.
dc.contributor.authorRicci, Giannina [UNIFESP]
dc.contributor.authorSano, A.
dc.contributor.authorYokoyama, K.
dc.contributor.authorOhori, A.
dc.contributor.authorKamei, K.
dc.contributor.authorFranco, Marcello Fabiano de [UNIFESP]
dc.contributor.authorMiyaji, M.
dc.contributor.authorNishimura, K.
dc.contributor.institutionChiba Univ
dc.contributor.institutionFujisawa Pharmaceut Co Ltd
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:37:07Z
dc.date.available2016-01-24T12:37:07Z
dc.date.issued2004-05-01
dc.description.abstractParacoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.en
dc.description.affiliationChiba Univ, Pathogen Fungi & Microbial Toxicoses Res Ctr, Chuo Ku, Chiba 2608673, Japan
dc.description.affiliationFujisawa Pharmaceut Co Ltd, Analyt Res Labs, Osaka, Japan
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med, Dept Anat & Patol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent93-97
dc.identifierhttp://dx.doi.org/10.1016/j.femsle.2004.03.015
dc.identifier.citationFems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.
dc.identifier.doi10.1016/j.femsle.2004.03.015
dc.identifier.issn0378-1097
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/27715
dc.identifier.wosWOS:000221218300013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFems Microbiology Letters
dc.rightsAcesso aberto
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectgp43 geneen
dc.subjectParacoccidioides brasiliensisen
dc.subjectparacoccidioidomycosisen
dc.titleDetection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) methoden
dc.typeArtigo
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