Physicochemical and chromatographic evaluation of benzhydrylamine-resin as an anion exchanger solid support

Physicochemical and chromatographic evaluation of benzhydrylamine-resin as an anion exchanger solid support

Author Carvalho, Regina Siqueira Haddad Autor UNIFESP Google Scholar
Nakaie, Clovis Ryuichi Autor UNIFESP Google Scholar
Silva, Elias Horacio da Autor UNIFESP Google Scholar
Santos, Isabel Anunciacao Neves dos Autor UNIFESP Google Scholar
Dietrich, Carl Peter von Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Tersariol, Ivarne Luis dos Santos Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Benzhydrylamine-resin (BHAR), a copolymer of styrene-divinylbenzene containing phenylmethylamine groups, used as solid support for peptide synthesis, was examined regarding physicochemical and anion exchanger chromatographic properties. the greater the ionic strength of the medium the poorer the solvation of beads. This effect is less pronounced the higher the amino group content of BHAR. the BHAR chromatographic behavior was compared with commercial cationic resins in columns of constant cation binding capacity. Three negatively charged heparan sulfate disaccharides were successfully purified in a 2.4 mmol/g BHAR that showed as good or better anion exchange performance than classical tertiary or quaternary amino group-containing resins. the BHAR chromatographic resin exclusion limit was estimated to be 30 kDa based on purification experiments of heparins of different molecular weight. (C) 2003 Elsevier Science B.V. All rights reserved.
Keywords Ion exchanger chromatography
Polymer
Disaccharide
Benzhydrylamine-resin
Benzydrylamine
Peptide
Language English
Date 2003-07-31
Published in Journal of Biochemical and Biophysical Methods. Amsterdam: Elsevier B.V., v. 57, n. 1, p. 75-83, 2003.
ISSN 0165-022X (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 75-83
Origin https://dx.doi.org/10.1016/S0165-022X(03)00104-0
Access rights Closed access
Type Article
Web of Science ID WOS:000184300900007
URI https://repositorio.unifesp.br/handle/11600/27326

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