DNA methylation, ageing and ribosomal genes activity

DNA methylation, ageing and ribosomal genes activity

Author Carvalho, C. V. de Google Scholar
Payao, SLM Google Scholar
Smith, M. D. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract DNA methylation is an epigenetic mechanism controlling of gene expression. We studied the effect of a demethylation agent 5-azacytidine, on ribosomal gene activity observed by Nucleolus Staining Giemsa in the chromosomes of eight Alzheimer's disease patients, eight elderly healthy individuals and eight young healthy controls. Metaphase cells were obtained from lymphocyte cultures. All the acrocentric chromosome pairs were analyzed using G banding followed by Nucleolus Staining Giemsa banding in the same cell. the Alzheimer's disease patients and elderly healthy control did not show a variation in the transcriptional activity of ribosomal genes with or without 5-azacytidine treatment, whereas the young control group showed an increase in this activity with the treatment.
Keywords ageing
ribosomal genes
Alzheimer's disease
DNA methylation
nucleolus organizer region
Language English
Date 2000-01-01
Published in Biogerontology. Dordrecht: Kluwer Academic Publ, v. 1, n. 4, p. 357-361, 2000.
ISSN 1389-5729 (Sherpa/Romeo, impact factor)
Publisher Kluwer Academic Publ
Extent 357-361
Origin http://dx.doi.org/10.1023/A:1026542618182
Access rights Closed access
Type Article
Web of Science ID WOS:000166618300007
URI http://repositorio.unifesp.br/handle/11600/26225

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