Transcriptional regulatory elements in the rat bradykinin B-2 receptor gene

Abstract

In an attempt to elucidate the mechanisms underlying the regulation of bradykinin B2 receptor gene expression, the molecular structure of the rat gene including the 5'-flanking region was characterized (J. Biol. Chem. 269: 26920-26925, 1994). in this study we show that the gene spans about 32 kb, including a long first intron of 25 kb. the promoter region drives reporter gene expression in NG108-15 neuroglioma cells, and its expression is upregulated by cAMP bradykinin, phorbol esters and by coexpression of an activated ras oncogene but not by dexamethasone.