PPG - Ecologia e Evolução
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Navegando PPG - Ecologia e Evolução por Assunto "16S rDNA"
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- ItemAnálise filogenética e cromossômica de escorpiões do "complexo" Tityus trivittatus,1898 (Buthidae)(Universidade Federal de São Paulo, 2017-03-23) Costa, Renata Clicia dos Santos [UNIFESP]; Schneider, Marielle Cristina [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The family Buthidae family is the largest within the order Scorpiones, with approximately 90 genera and 1,100 species. The genus Tityus is the richest, including more than 220 species distributed in Central and South America. Based on morphological characteristics, the species of the genus Tityus are grouped into subgenera, groups or complexes. One of these complexes is Tityus trivittatus, composed by four species, namely: T. charreyroni, T. dorsomaculatus (= T. costatus), T. fasciolatus and T. trivittatus. However, no study to test the monophyly of T. trivittatus “complex” was accomplished. Moreover, the authors have discussed about the taxonomic validity of certain species of this complex. In this work, we test the monophyly of trivittatus “complex”, aiming to understand the relationship between the species using from molecular data and cytogenetic information. In addition to representatives of trivittatus complex, other morphologically related species were used in analysis, T. carvalhoi, T. confluens, T. aff. trivittatus, and Rhopalurus rochai. This last species was used as outgroup. Molecular analyzes were performed with the mitochondrial 16S rDNA. For karyotype studies, we established the diploid number and location of the nucleolar organizer regions (NORs). Our results revealed that trivittatus “complex” is not monophyletic, with T. costatus (maculated and trifasciated forms) and T. fasciolatus nested in A clade, and T. trivittatus, T. confluens, T.aff. trivittatus, T. carvalhoi and T. charreyroni, in B clade. The cytogenetic data were congruent with phylogenetic hypothesis, i.e., all species of the A clade showed the highest diploid numbers, ranging from 2n=14 to 2n=24, whereas those of the B clade exhibited lowest diploid numbers, varying from 2n=5 to 2n=7. Despite these differences in diploid number, all species revealed two NORs localized on terminal region of the chromosomes.