Navegando por Palavras-chave "neovascularization"

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    The effects of the subconjunctival injection of bevacizumab (Avastin®) on angiogenesis in the rat cornea
    (Academia Brasileira de Ciências, 2007-09-01) Barros, Luiz F.m.; Belfort, Rubens Junior [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    The purpose of this study was to evaluate the effects of the use of the subconjunctival injection of bevacizumab (Avastin®) on angiogenesis in the rat cornea. Corneas of 20 Wistar male rats were cauterized with silver nitrate crystal. Animals were divided in four groups: control group (GC) that received subconjunctivally 0.02 ml of 0.9% saline solution on the day of the lesion; group GO that received subconjunctivally 0.02 ml of bevacizumab just after the lesion; group G3 that received bevacizumab on day 3 and group G5 that received bevacizumab on day 5 after lesion. Animals were euthanized on day 7. The newly formed vessels were quantified after China Ink perfusion and photographs were obtained and analyzed in a computerized system (Image Pro-Plus®). In the control group, neovascularization covered 53.56% ± 15.11 (mean ± SD) of the corneal surface, compared with 35.57% ± 18.80 (mean ± SD) in the G0 group, 30.60%±11.82 (mean±SD) in the G3 and 35.86%±0.07 (mean±SD) in the G5. The results showed an inhibition of angiogenesis when the control group was compared with all treated groups. These results suggest that subconjunctival injection of bevacizumab is able to inhibit corneal angiogenesis independently of the day of treatment.
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    Vascular Therapy for Radiation Cystitis
    (Wiley-Blackwell, 2011-01-01) Soler, Roberto [UNIFESP]; Vianello, Alberto; Fuellhase, Claudius; Wang, Zhan; Atala, Anthony; Soker, Shay; Yoo, James J.; Williams, James Koudy; Wake Forest Univ Hlth Sci; Ludwig Maximilians Univ Munchen; Univ Perugia; Universidade Federal de São Paulo (UNIFESP)
    Purpose: the underlying pathology of radiation cystitis is cellular and vascular damage followed by increased fibrosis and inflammation. This study was to determine if neovascular- promoting therapy could reduce the pathological changes in the bladder wall associated with pelvic irradiation. Methods: Adult female Lewis inbred rats were irradiated with a single dose of 20 Gy directed at their bladder. Four weeks later, 30 rats were divided equally into one of three treatment groups for bladder wall injection of: (1) PBS (Control); (2) PBS containing 50 ng vascular endothelial growth factor (VEGF165); or (3) PBS containing 1 x 10(6) rat endothelial cells (EC). Age- matched non- irradiated rats (n 10) served as untreated controls. At either 1.5 or 3 months following radiation, bladders were analyzed for collagen deposition using Masson's Trichrome staining of collagen and muscle and vascularization using Von Willebrand factor staining of ECs. Quantitative- PCR was used to examine markers of angiogenesis, hypoxia, and fibrosis. Results: the collagen/ muscle ratio was doubled in the control group 3months post- irradiation (P < 0.05 vs. non- irradiated bladders). Both ECs and VEGF inhibited increases in collagen content (P < 0.05 vs. control). Similarly, irradiation reduced bladder wall vessel counts compared to non- irradiated controls (P < 0.05) and both ECs and VEGF maintained vessel counts similar to that of non- irradiated controls (P < 0.05). PCR analysis showed a higher expression of neovascular markers (CD31, KDR) in the EC and VEGF groups compared to non- irradiated controls (P < 0.05). Conclusions: Angiogenesis therapy may be useful in the prevention and/or treatment of the underlying pathology of radiation cystitis. Neurourol. Urodynam. 30: 428- 434, 2011. (C) 2010 Wiley-Liss, Inc.