Navegando por Palavras-chave "graft-versus-host disease"

Agora exibindo 1 - 3 de 3
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Avaliação da Barreira Hemato-Encefálica no transplante de medula óssea
    (Assoc Arquivos De Neuro- Psiquiatria, 1997-12-01) Almeida, Sergio Monteiro de [UNIFESP]; Livramento, Jose Antonio; Pasquini, Ricardo; Palou, Viviana Boccardi; Oliveira, Aparecido Mendes de; Doi, Elvira Missako; Ono, Margaret; Aso, Mirna Cigemi; Ferreira, Euripedes [UNIFESP]; UNIV FED PARANA; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    The blood-brain barrier (BBB) contributes to the central nervous system (CNS) immunological isolation. BBB has never been studied in patients who developed chronic graft-versus-host disease (GVHD) after allogeneic bone marrow transplants (BMT), from HLA identical related donors. BB disruption was investigated through the cerebrospinal fluid (CSF) proteins, quantitative and graphically, in order to detect the incidence and possible pathophysiology of the CNS involvement in chronic GVHD. Thirty three CSF and matched serum samples from chronic myeloid leukemia patients were collected pre BMT, pos BMT and during chronic GVHD. There was no evidence of BBB disruption in any patient studied.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Avaliação da síntese intratecal de imunoglobulinas no transplante de medula óssea
    (Assoc Arquivos De Neuro- Psiquiatria, 1997-12-01) Almeida, Sergio Monteiro de [UNIFESP]; Livramento, Jose Antonio; Pasquini, Ricardo; Palou, Viviana Boccardi; Oliveira, Aparecido Mendes de; Doi, Elvira Missako; Ono, Margaret; Aso, Mirna Cigemi; Ferreira, Euripedes [UNIFESP]; UNIV FED PARANA; Universidade Federal de São Paulo (UNIFESP); Universidade de São Paulo (USP)
    The central nervous system involvement in chronic graft versus host disease (GVHD) has been suggested. Chronic GVHD resembles auto immune connective tissue disorders. In order to investigate the immunoglobulin intra blood brain barrier (BBB) synthesis during chronic GVHD, and contribute to understanding the pathophysiology of the disease, we studied 33 patients who underwent allogeneic bone marrow transplants (BMT) from HLA identical related donors. Immunoglobulin intra BBB synthesis was investigated quantitative and qualitatively. The samples were collected pre BMT, pos BMT and during chronic GVHD. There were no evidence of immunoglobulin intra BBB synthesis, and no oligoclonal bands were found. Only isolated cases suggested IgG and IgA intra BBB synthesis, and in one case IgM during GVHD.
  • Nenhuma Miniatura disponível
    Item
    Somente Metadadados
    Cell populations and adhesion molecules expression in conjunctiva before and after bone marrow transplantation
    (Elsevier B.V., 2005-09-01) Rojas, B.; Cuhna, R.; Zafirakis, P.; Ramirez, J. M.; Lizan-garciia, M.; Zhao, T.; Foster, C. S.; Univ Complutense Madrid; Harvard Univ; Universidade Federal de São Paulo (UNIFESP); Hosp Gen Albacete
    We were interested to analyse the composition of the cellular infiltrate and adhesion molecules expression in the conjunctiva before and at least one hundred days after autologous and allogenic bone marrow transplantation (BMT) and its relation with the presence of dry eye. We used immunohistochemistry on cryopreserved human conjunctiva with monoclonal antibodies to T-lymphocytes (CD3, CD4 and CD8), B-lymphocytes (CD19), macrophages (CD14), natural killer cells (NK, CD57), intercellular adhesion molecule 1 (ICAM-1), E-selectin, vascular cell adhesion molecule-1 (VCAM-1), lymphocyte function associated antigen-1 (LFA-1), very late antigen-4 (VLA-4), interleukin 2 receptor (IL2r, CD25) and HLA-DR. Our autologous recipients had no graft-versus-host disease (GVHD) but allogenic patients had chronic GVHD. After autologous BMT the conjunctiva had significantly more: (1) T lymphocytes (CD3+, CD4+, CD8+) in the epithelium; (2) CD4+ and CD 14+ cells in the stroma; and (3) VLA-4 expression in the stroma than before BMT. After allogenic BMT, the conjunctiva exhibited a significant increase of: (1) CD3+ and CD 14+ cells in the epithelium; (2) T lymphocytes (CD3+, CD4+, CD8+) and CD 14+ cells in the stroma; and (3) VLA-4 and LFA-1 expression in the stroma than before BMT. After the engraftment, the comparison between autologous and allogenic recipients revealed that: (1) there were no significant differences in adhesion molecule expression; (2) the epithelium of autologous recipients had significantly more CD3+ cells; and (3) the stroma of allogenic patients had significantly more CD3+ and CD8+ cells. Among allogenic recipients, CD14+ cells were significantly increased both in the epithelium and in the stroma of patients with signs or symptoms of dry eye in comparison with patients without ocular involvement. Additionally, those having keratoconjunetivitis sicca (KCS) had CD4/CD8 ratios significantly higher than those without KCS.In conclusion, in the conjunctiva after autologous BMT a subclinical cell mediated immune reaction seems to take place. the conjunctivitis of chronic GVHD is complex, with T cells and macrophages dramatically contributing to the process. (c) 2005 Elsevier B.V. All rights reserved.