Navegando por Palavras-chave "epithelial cell"

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    Scanning electron microscopic study of the dorsal surface of the tongue of Calomys callosus mouse
    (Gustav Fischer Verlag Jena, 1995-11-01) Utiyama, Cássia; Watanabe, Ii-sei; Konig, Bruno; Koga, Luciane Y.; Semprini, Marisa; Tedesco, Roberto Carlos [UNIFESP]; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP)
    The characteristics of the dorsal mucosal surface of Calomys callosus' tongue were studied employing scanning electron microscopy. The samples were fixed with modified Karnovsky solution, and postfixed in 1% OsO4, followed by dehydration and critical point drying. The scanning electron microscopy gives a three dimensional image of the filiform, fungiform, vallate and foliate papillae. The filiform papillae were found on the anterior tip and dorsal surface; the fungiform papillae on the dorsal and anterior surfaces; only one vallate papilla, elongated in shape, is located in the posterior third, and the small foliate papillae were to be seen in the posterolateral region of the tongue.
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    Structural characterization of the capybara (Hydrochaeris hydrochaeris) tongue by light, scanning, and transmission electron microscopy
    (Wiley-Blackwell, 2013-02-01) Watanabe, Ii-Sei; Dos Santos Haemmerle, Carlos Alexandre; Dias, Fernando Jose; Cury, Diego Pulzatto; Pereira Da Silva, Marcelo Cavenaghi [UNIFESP]; Kronka Sosthines, Marcia Consentino; Dos Santos, Tatiana Carlesco; Guimaraes, Juliana Placido; Miglino, Maria Angelica; Universidade de São Paulo (USP); Universidade Federal de São Paulo (UNIFESP); Univ Maringa
    Capybara is the largest rodent in the world and displays a seasonally dependent herbivore feeding behavior. Here, we present an anatomical contribution for understand this fact, by light, scanning, and transmission electron microscopy methodologies for tongue tissue analysis. the histological preparations revealed filiform, fungiform, vallate, and foliate papillae on the dorsal mucosa of the capybara tongue. the epithelial layer exhibited a lining of keratinized stratified squamous epithelial cells. the lamina propria was characterized by a dense connective tissue composed of the primary and secondary papillar projections. We also revealed the original aspects of the connective papillae. the shapes of the papillae varied by region of the tongue, and filiform, fungiform, vallate, and foliate papillae and subjacent layers of muscular fibers were observed. Pyriform taste buds occupying the epithelial layer of fungiform, vallate and foliate papillae were identified and the intracellular components of the taste buds and the intracorpuscular amyelinated nerve fibers were observed. the taste buds were characterized by the distribution of granular endoplasmic reticulum throughout the perinuclear area, the Golgi apparatus, and mitochondrial assemblies of various distinct diameters. Mitochondrial accumulation was also observed in the collagen bundle-surrounded amyelinated nerve fibers beside the basal cells. Therefore, these peculiar anatomical descriptions may contribute to understanding the adaptation of the feeding behavior of capybaras in a seasonally changing environment. Microsc. Res. Tech., 2013. (C) 2012 Wiley Periodicals, Inc.
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    Ultrastructure of the adhesion of bacteria to the epithelial cell membrane of three-day postnatal rat tongue mucosa: a transmission and high-resolution scanning electron microscopic study
    (Fundação Odontológica de Ribeirão Preto, 2007-01-01) Watanabe, Ii-sei; Ogawa, Koichi; Silva, Marcelo Cavenaghi Pereira da [UNIFESP]; Motoyama, Aracy Akiko; Kado, Eduardo Shigueaki; Lopes, Ruberval Armando; University of São Paulo Institute of Biomedical Sciences Department of Anatomy; Fukuoka University Department of Anatomy; Universidade Federal de São Paulo (UNIFESP); University of São Paulo School of Dentistry of Ribeirão Preto Department of Morphology and Stomatology
    Togue mucosa surface of 3-day postnatal rats was examined under transmission electron microscopy (TEM) and high-resolution scanning electron microscopy (HRSEM). For HRSEM analysis, the specimens were fixed in the same solution for 24 h, postfixed in 2% osmiun tetroxide, critical-point dried and coated with platinum-palladium. For TEM analysis, the specimens were fixed using modified Karnovsky solution and embedded in Spurr resin. The results revealed the presence of numerous microplicae in the membrane surface of keratinized epithelial cells to which groups of bacteria were attached. These bacteria were staphylococcus and coccus organized either in rows or at random, which were visualized in three-dimensional HRSEM images. At high magnification, the TEM images revealed the adhesion of bacteria to the cell membrane through numerous filamentous structures comprising the glycocalyx. The fine fibrillar structures rising from each bacterium and from cell membrane were clearly seen. These characteristics on bacteria structure may be used for future control or prevention of bacterial diseases and for installation of the oral native flora.