Navegando por Palavras-chave "cox-2"

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    Avaliação de genotoxicidade e expressão proteíca de cox-2, inos e tnf-alfa, após tratamento com suco de uva concentrado (g8000) na colite experimental induzida pelo 2,4,6 - ácido trinitrobenzeno-sulfônico (tnbs) em ratos wistar
    (Universidade Federal de São Paulo (UNIFESP), 2014-03-17) Marchi, Patricia [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Este trabalho teve como objetivo avaliar os compostos fenólicos presentes no suco de uva perante a redução dos efeitos nocivos induzidos pela colite experimental. Material e Métodos: Um total de 42 ratos machos Wistar foram distribuídos aleatoriamente em sete grupos: grupo G1-Sham: controle solução salina; G2 – TNBS/etanol: controle TNBS (50 mg/kg); G3 - controle suco de uva a 2%; G4 – TNBS/etanol tratado com suco de uva a 1% após 24h da indução da colite; G5 – TNBS/etanol tratado com suco de uva a 1% a partir do 7 º dia após a indução da colite; G6 – TNBS/etanol tratado com suco de uva a 2% após 24h da indução da colite; G7 – TNBS/etanol tratado com suco de uva a 2% a partir do 7 º dia após a indução da colite. Todos os animais foram eutanasiados no 16º dia, após o início do experimento. Resultados: A imunoexpressão do TNF-alfa e iNOS reduziu após o tratamento com suco de uva concentrado (G8000™) 24h ou sete dias após a indução da colite em todas as doses testadas. A imunoexpressão de COX-2 diminuiu nos grupos tratados com suco de uva a 1%. O suco de uva (G8000™) a 1% nos últimos sete dias de tratamento, bem como a 2% reduziu a genotoxicidade em sangue periférico. Conclusão: Em suma o suco de uva concentrado (G8000™) principalmente na dose de 1% exerce efeitos anti-inflamatórios na colite crônica causada pelo TNBS como resultado da modulação da expressão de citocinas pró-inflamatórias e redução da genotoxicidade em céluas de sangue periférico
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    Característica do processo de regeneração de músculos esqueléticos de diferentes origens embrionárias em camundongos mdx submetidos ao treinamento físico aeróbio
    (Universidade Federal de São Paulo (UNIFESP), 2016-03-18) Lazzarin, Mariana Cruz [UNIFESP]; De Oliveira, Flavia [UNIFESP]; http://lattes.cnpq.br/3387760393535776; http://lattes.cnpq.br/1579497511371132; Universidade Federal de São Paulo (UNIFESP)
    Duchenne muscular dystrophy (DMD) is a genetic disorder that generates loss of functionality, with impaired strength and muscle mass. The effect of exercise on muscle dystrophic is not well established. Thus, the aim of this study was to analyze the effects of aerobic exercise training on the morphology and myogenic markers of muscle regeneration and inflammation of skeletal muscles from different embryonic origins in C57BL/10-Dmdmdx mice. Were used male C57BL/10 mice (n = 10) and C57BL/10-Dmdmdx (n = 10), with eight weeks old. They were distributed into four groups (n = 5): Sedentary Control (SC), Trained Control (TC), Sedentary Mdx (S-Mdx) and Trained Mdx (T-Mdx). The trained groups were submitted to seven weeks of aerobic exercise, five times weekly at a high intensity speed for 60 minutes with treadmill downward of -15°. After euthanasia, the gastrocnemius muscles (somitic embryonic origin) and masseter (branchiomeric embryonic origin) were dissected and processed for undergoing: Histopathological; morphometric (cell density of transverse sections and the cross-sectional area of muscle fibers); immunohistochemistry markers for muscle regeneration (MyoD, and Myogenin), inflammation (COX-2) and oxidative stress (8-OHdG). Histopathological results showed morphological changes in Mdx groups as well as the quantification of connective tissue, both in the gastrocnemius muscle and masseter. The morphometric analysis revealed that Mdx groups (S-Mdx and T-Mdx) have heterogeneity in the muscle fibers and the gastrocnemius muscle when compared with masseter muscle, which had a similar pattern to the control groups (SC and TC). Immunohistochemical results showed less nuclear staining MyoD and Myogenin for Mdx groups (SMdx and T-Mdx) when compared with control groups (SC and TC) for gastrocnemius muscle. Masseter muscle, differed between Mdx and Control groups about Myogenin parameter. In both muscles, the Mdx-T group had higher percentage of immunoreactive nuclei Myogenin compared to Mdx-S. Immunohistochemical results of COX-2 were higher for Mdx groups in both muscles, but only the gastrocnemius muscle was influenced training. The immunoreactivity of 8-OHdG showed statistical significance only about physical training and in gastrocnemius muscle. The conclusion of the study showed that the embryonic origin of skeletal muscle influenced the response of muscular dystrophy and exercise. Physical training protocol modified the histopathology and the presence of connective tissue in the masseter muscle. In addition, physical training interfered in increased immunoreactivity of COX-2 in the gastrocnemius muscle dystrophic. Furthermore, it was able to increase Myogenin in both muscles and groups.
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    Estudo histológico da cicatrização da parede abdominal em camundongos com a neoplasia de ehrlich por método imuno-histoquímico
    (Universidade Federal de São Paulo (UNIFESP), 2014-08-27) Salgado, Flavio Luiz Lima [UNIFESP]; Lopes Filho, Gaspar de Jesus Lopes Filho [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To evaluate the histological changes in laparotomy scars of healthy BALB/c mice with an Ehrlich tumor in its various forms of presentation using immunohistochemical techniques. Methods: Studies were conducted on 54 mice divided into 3 groups of 18 animals. One group was the control, the second presented the Ehrlich ascites tumors, and the third group had the subcutaneous form. Seven days after inoculation of the tumor, all 54 mice were submitted to laparotomy. All of the animals in the experiment were operated on again on the seventh day after surgery, with resection of the scar and subsequent euthanasia of the animal. The scars were sent for histological assessment using immunohistochemical techniques to evaluate Cox-2 (cyclooxygenase 2), VEGF (vascular endothelial growth factor) and FGF (fibroblast growth factor); and were analyzed semi-quantitatively on the laparotomy scar and the abdominal wall farthest from the site of the operation. Results: Assessing the weight of the animals, the correct inoculation of the tumor and weight gain in the group with ascites tumors was observed. The histological studies showed that groups with the tumor showed a statistically significant higher presence of Cox-2 compared to the control. In the Cox-2 study of the abdominal wall location, this was the site in which the ascites group showed the most significant difference. The VEGF did not present any significant differences between the 3 groups, regardless of the site studied. The FGF showed a significant increase in animals with the tumor. Conclusion: In mice with different forms of presentation of the Ehrlich tumor, there was a statistically significant predominance of immunoreactivity of Cox-2 and FGF in the surgical scars compared to the group of healthy animals. VEGF showed no significant difference. This finding suggests more inflammation and fibroblast proliferation in animals from the groups with tumors.
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    Imunoexpressão dos biomarcadores ts, cox-2, egfr, msh6, mlh1 no adenocarcinoma colorretal e sua correlação com o grau de diferenciação tumoral e os fatores prognósticos
    (Universidade Federal de São Paulo (UNIFESP), 2013-09-25) Batista, Wilson Roberto [UNIFESP]; Matos, Delcio Matos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Objective: To study the immunoexpression of biomarkers TS, p53, COX2, EGFR, MSH6, MLH1 in patients with colorectal carcinoma, correlating with the degree of tumor differentiation and clinical prognostic factors patolólogicos. Methods: We analyzed tissues fixed in formalin and embedded in paraffin blocks of tumors from 107 patients, for immunohistochemistry by the streptavidin-biotin method using the technique of matrix arrangement of tissue samples (tissue-microarray). In the evaluation of positive markers was used categorical scores that determined the cutoff value in the percentage of stained tumor cells. Tissue expression of the proteins were correlated with the variables of degree of cell differentiation, staging, disease-free, recurrence, survival and specific mortality. We employed the Fisher exact test (Agresti, 1990) to study the association between tumor grade and TS, Cox-2, EGFR, MSH1, MSH6 and p53, and tumor staging correlated with TS, Cox-2, EGFR, MSH1, MSH6 and p53. Estimation of Kaplan-Meier (Collett, 2003), Log-rank test (Collett, 2003) and adjusting the Cox regression model (Cox, 1972) to investigate the behavior of the overall survival of patients (months) according to TS, COX-2, EGFR, MSH6, MLH1 and p53 and disease-free interval of subjects (months), according to TS, COX-2, EGFR, MSH6, MLH1 and p53. Results: The degree of tumor differentiation of individuals is not associated with TS (p = 0.138), COX-2 (p = 0.428), EGFR (p = 0.103), MSH6 (p = 0.876), MLH1 (p = 0.792) and p53 (p = 0.884). The staging is not associated with TS (p = 0.817), COX-2 (p = 0.842), EGFR (p = 0.344), MSH6 (p = 0.923) and p53 (p = 0.666). The same behavior was not observed for MLH1 (p = 0.021) in which the group of patients with stage III or IV is a higher percentage of MLH1 negative (27.4%) than in the group of patients with stage 0, I, or II (2.2%). The survival time of individuals is not related to TS (p = 0.480), COX-2 (p = 0.998), EGFR (p = 0.600), MSH6 (p = 0.318), MLH1 (p = 0.798) and p53 (p = 0.695). The disease-free interval of subjects is not related to TS (p = 0.356), COX-2 (p = 0.885), EGFR (p = 0.786), MSH6 (p = 0.178), MLH1 (p = 0.691) and p53 (p = 0.441). Conclusion: There was no correlation of the association between the degree of tumor differentiation, staging, survival time and disease-free interval with markers TS, p53, COX2, EGFR, MSH6. In advanced cases of RCC with stage III and IV, there was a higher percentage of MLH1 negative.
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    Investigação da proteína anti-inflamatória anexina a1 em culturas de explantes de vilos placentários humanos infectados por toxoplasma gondii
    (Universidade Federal de São Paulo (UNIFESP), 2016-01-29) Oliveira, Marystela Favero de [UNIFESP]; Oliani, Sonia Maria Oliani [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)
    Introduction: Inplacental developmenttheinvasive processes, differentiation and adaptationthat occurin the bodyare intense.Some complicationsare related toinadequate developmentof the placenta, such as increased expression ofTh2 cytokineswhich can causeincreased susceptibilityto toxoplasmosis and consequently, placentaland fetalinfection.Several investigationsindicate that among theanti-inflammatory actions of the proteinannexinA1(ANXA1) isthe reduction ofcytokinelevels. This proteinhas also been linkedwithproliferativeandapoptotic processes, andstudies indicatethat their actionsmay be mediatedbyreceptors such asformylatedpeptides (FPR). Although theanti-inflammatory effects of ANXA1and its Ac2-26peptidehave beenexplored inseveral investigations, their activitiesduringplacental developmentare not yet fullyclarified.Aims- ANXA1 was evaluated in this studyin culture explants of humanplacentalvillous in thirdtrimester of pregnancy, infected or notwithToxoplasmagondii. Additionally,post-infected explants weretreated with theAc2-26peptide. Methods ?The explantswere cultured insupplementedRPMI, incubated at37oC in5% CO2 incubatorand afterwands, either infectedor not withT.gondiiRH strain. After 48 hoursof infection, samples were treated with theAc2-26peptide for 24 hours,fixed, embedded in paraffinandhistologically examined. Placentalvillousfromexplants were analyzed for immunohistochemicalto verify the expressions of ANXA1, formyl peptide receptors (FPR1 and FPR2) and COX-2 in placentas infected or not with T. gondii.Placentas from third trimester were treated with peptide Ac2-26.Gene expression of ANXA1 was also evaluated in treated samples by real time PCR.Theparasitism rate by?-galactosidase colorimetric assayand the levels ofPGE2by ELISA was detected.Results-Expressions ofANXA1protein are coincident with the FPR1receptorin trophoblasticofcontrol andinfectedgroups.After Ac2-26peptidetreatment,the intracellular parasites decrease and ANXA1 expression increase insyncytiotrophoblast cells. The expression ofCOX-2andPGE2 levelswere lower in the infected group compared with respective uninfected group without treatment. Conclusion -Together, our data highlight that Ac2-26 peptide is able to induce an anti-parasitic effect in placental explants of the advanced gestational age.In this context, the major indication isthat FPR1 receptor, COX-2 and PGE2 levels may be involved in thecontrol mechanism of T. gondiiinfections