Navegando por Palavras-chave "bcl-2"
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- ItemSomente MetadadadosAnálise do tempo de sobrevida de doentes com adenocarcinoma colorretal esporádico pela utilização de um painel de biomarcadores de carcinogênese constituído por vegf, egfr, ki-67, p53 e bcl-2(Universidade Federal de São Paulo (UNIFESP), 2014-12-17) Luderer, Loreley Andrade [UNIFESP]; Matos, Delcio Matos [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Objective: To evaluate the prognostic power of survival of a carcinogenesis biomarkers panel formed by p53, VEGF, Bcl-2, Ki-67, and EGFR in subjects with sporadic colorectal adenocarcinoma subjected to radical surgical treatment. Methods: 114 post-surgical subjects with colorectal adenocarcinoma were studied and followed for 3 to 5 years at Fundação Pio XII – Hospital de Câncer de Barretos. The study was conducted in paraffin-embedded tumor tissue whose slides were stained using the hematoxylin-eosin technique. The tissue microarray slides, as well as the immunohistochemical staining, were examined by two pathologists, blinded to the evaluations. The statistical analyses were conducted using mean, median, minimum, maximum, and number of valid observations for the descriptive analysis of the numeric variable, global survival. The comparison of the expression of EGFR, VEGF, Ki-67, p53, and Bcl-2 biomarkers was conducted through the Chi-square test or, when required, Fisher’s exact test. The Cox regression model was used for global survival analysis with a panel of markers and for uni and multivariate global survival analyses. Results: Isolated expression correlation results of the markers with the variables: age, differentiation degree, venous invasion, perineural invasion, TNM (I+II) x (III+IV), and survival showed statistically significant differences in the EGFR expression with venous invasion, TNM classification, and global survival; the expression of the VEGF marker has showed significant correlation with the perineural invasion; the Ki-67 marker, with age, venous invasion, and TNM; expression of p53 was significantly related with age, venous invasion, TNM, and global survival; the Bcl-2 marker did not show significant correlation with any of the variables analyzed. The survival analysis, using the markers panel, has significantly showed lesser time of survival in surgical species with 60% or more overexpression. Conclusion: Overexpression of the selected tumor markers panel is related with lesser time of survival in those suffering from sporadic colorectal adenocarcinoma subjected to radical surgical treatment.
- ItemSomente MetadadadosBcl-2 oncogene expression in estrogen receptor-positive and negative breast carcinoma(I R O G Canada, Inc, 2008-01-01) Santos, L. G. dos; Lopes-Costa, P. V.; Santos, A. R. dos; Facina, Gil [UNIFESP]; Silva, B. B. da; Univ Fed Piaui; Universidade Federal de São Paulo (UNIFESP)Purpose: The aim of this study was to evaluate Bcl-2 oncogene expression in estrogen receptor (ER)-positive and negative breast carcinomas. Methods: A Study involving 72 cases of infiltrating ductal carcinoma of the breast in postmenopausal women divided into two groups: Group A (ER positive, n = 37) and Group B (ER negative, n = 35). Immunohistochemical analysis of bcl-2 expression was carried out semiquantitatively based on the percentage of stained tumoral cells and the intensity of staining. The chi-square test was used in the statistical analysis of the data and significance was established at p < 0.05. Results: Bcl-2 oncogene expression was statistically greater in tumors of Group A (59.5%) compared to those of Group B (8.6%), (p < 0.001). Conclusion: Bcl-2 had a significantly greater expression in the ER-positive breast tumors compared to ER-negative tumors.
- ItemSomente MetadadadosCell-Cycle Analysis and Apoptosis-associated Proteins in Cervical Lesions of Brazilian Women(Int Inst Anticancer Research, 2014-06-01) Dobo, Cristine [UNIFESP]; Oshima, Celina Tizuko Fujiyama [UNIFESP]; Lima, Flavio de Oliveira [UNIFESP]; Gomes, Thiago Simao [UNIFESP]; Stavale, Joao Norberto [UNIFESP]; Arias, Vitor [UNIFESP]; Ribeiro, Daniel Araki [UNIFESP]; Focchi, Gustavo Rubino de Azevedo [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Aim: The aim of the present study was to detect the relative expressions of p53, p21(Waf1/Cip1), p27(Kip1) Bcl-2 and cleaved caspase-3 in cervical lesion samples from Brazilian women by immunohistochemistry. Materials and Methods: A total of 230 cervical biopsies in paraffin-embedded blocks were studied: 43 were invasive squamous cell carcinomas (SCC), 52 carcinomas in situ/cervical intraepithelial neoplasias III (CIN III), 54 cervical intraepithelial neoplasias II (CIN II), 51 cervical intraepithelial neoplasias I (CIN I) and 30 non-neoplastic lesions (NN) with benign cellular changes. Results: Significant differences were observed in the p53 expression between the different groups: NN and CIN I (p=0.010); NN and CIN II (p<0.00001); CIN II and CIN III (p=0.02); CIN II and CIS (p=0.0220); CIN II and CEC (p=0.010). Regarding p21(WAF1/Cip1), significant differences were observed between NN and CEC (p=0.001); CIN I and CEC (p=0.001); CIN II and CIN III (p=0,001); CIN II and CIS (p=0.0004) and CIN II and CEC (p<0.0001). For p27(Kip1), significant differences were observed between NN and GIN I (p<0.00001); NN and CIN II (p<0.00001); NN and CIS (p=0.038); CIN I and CIN III (p=0.001); CIN I and CIS (p=0.009); CIN I and CEC (p=0.0001); CIN II and CIN III (p=0.000.3); CIN II and CIS (p=0.002); CIN II and CEC (p< 0.00001). Bcl-2 and caspase-3 did not show remarkable differences between groups. Conclusion: p53, p21(WAF1/CIP1), p27(KIP1) appear to be involved in the course of carcinogenesis. Rare expression of Bcl-2 and cleaved caspase-3 suggests that these proteins probably do not participate in cervical apoptosis.
- ItemAcesso aberto (Open Access)Comparison of positivity frequency of bcl-2 expression in prostate adenocarcinoma with low and high Gleason score(Associação Paulista de Medicina - APM, 2001-07-01) Hering, Flavio Luis Ortiz [UNIFESP]; Lipay, Mônica Vannucci Nunes [UNIFESP]; Lipay, Marco Aurélio Silva [UNIFESP]; Rodrigues, Paulo Roberto Teixeira [UNIFESP]; Nesralah, Luciano José [UNIFESP]; Srougi, Miguel [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)CONTEXT: Multiple genetic and epigenetic factors have been implicated in the oncogenesis and progression of prostate cancer. The major difficulty is in that the clinical management stems from the reality that reliable and accurate prognostic biomarkers are not available and that effective treatment regimens forming hormone-resistant prostate cancers are yet to be developed. Among the most important regulators of apoptosis and programmed cell death is the bcl-2 gene and its related proteins. Elevated levels of bcl-2 protein may contribute to the progression of prostate cancers to a metastatic and hormone-insensitive state characterized by poor responses to chemotherapy. OBJECTIVE: To characterize the expression of bcl-2 proteins as a prognostic factor in humans. DESIGN: A retrospective approach. SETTING: Urology section, Federal University of São Paulo. DIAGNOSTIC TEST USED: Immunohistochemical analysis using bcl-2 protein antibody and normal staining by hematoxylin-eosin. MAIN MEASUREMENTS: Prognostic relations and protein expression were evaluated considering the total sample (28) divided into two groups, high (8 to 10) and low (2 to 4), separated according to the histological differentiation grade (Gleason score) with 10 and 18 samples, respectively. RESULTS: The differentiation of grade into two groups separated according to the Gleason score in low and high types presented different bcl-2 expression (P < 0.001). CONCLUSION: The higher frequency of bcl-2 immunostaining in tumor samples was observed in association with more advanced Gleason scores and suggests that an increase in the ratio of this anti-apoptotic protein often occurs during progression of prostate cancers.
- ItemSomente MetadadadosGemistocytes in astrocytomas: Are they a significant prognostic factor?(Springer, 2006-10-01) Martins, Dely C.; Malheiros, Suzana M.; Santiago, Lucila H.; Stavale, Joao N.; Universidade Federal de São Paulo (UNIFESP)Our aim was to retrospectively evaluate the influence of gemistocytic astrocytes, cellular proliferation indices, immunoexpression of proteins p53 and bcl-2 in the clinical outcome of 39 patients with WHO grade II and III astrocytomas with the presence of gemistocytes. the mean proportion of gemistocytes was 18.7% and the mean proliferative index was 3.3%. Immunoexpression of p53 was detected in 29 cases (74.4%) and all cases (100%) were positive for bcl-2. the median overall survival was 97.2 months and the progression-free survival was 43.1 months. Estimated 1-, 5- and 10-year overall survival rates were 94.3%, 69.5% and 46.4%; 1-, 5- and 10-year progression-free survival rates were 91.1%, 26.1% and 13.1%. Out of 24 who presented clinical and neuroimaging worsening, characterized as tumor progression or recurrence, 16 had histological confirmation and were also analyzed. We could not detect significant differences when comparing all the indices between WHO grade II and III and also between the first and second biopsies. We also could not detect significant differences in progression-free and overall survival when analyzing the gemistocyte index and the immunohistochemical labeling indices p53, bcl-2 and MIB-1, as well as patients' age (median value, up to 34 vs. over 34 years) and histological grade (II or III). Our finding confirms recent reports that question the role of gemistocytes as a prognostic factor in diffuse astrocytomas. the significance and role of gemistocytes in astrocytomas has yet to be defined and warrants further study.
- ItemSomente MetadadadosIncrease in bax expression and apoptosis are associated in Huntington's disease progression(Elsevier B.V., 2008-06-13) Teles, A. V. F. F. [UNIFESP]; Rosenstock, T. R. [UNIFESP]; Okuno, C. S. [UNIFESP]; Lopes, G. S. [UNIFESP]; Bertoncini, C. R. A. [UNIFESP]; Smaili, Soraya Soubhi [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)Huntington's disease (HD) is a hereditary dominant neurodegenerative disorder and the progression of the disease may be associated with apoptosis and altered expression of apoptotic proteins. the aim of this study was to investigate gene expression of bax and bcl-2 in tissues from R6/1 transgenic (TGN) mice of different ages (3, 6 and 9 months). the mRNA expression was investigated and related to apoptotic cells measured by TUNEL Results showed a significant and progressive increase in box levels in the cortex of TGN (from 10 to 33%) when compared to control (CT) (8 to 20%) mice with 3, 6 and 9-month-old. the increase in box was correlated with the elevation in the number of apoptotic nuclei, especially in the cortex of 6 (10%) and 9 (18%)-month-old mice. Increase in bax expression might be related to an apoptotic induction which contributes to the HD progression. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
- ItemSomente MetadadadosSleep deprivation does not affect indices of necrosis or apoptosis in rat brain(Taylor & Francis Ltd, 2002-02-01) Hipólide, Débora Cristina [UNIFESP]; D'Almeida, Vania [UNIFESP]; Raymond, Roger; Tufik, Sergio [UNIFESP]; Nobrega, J. N.; Ctr Addict & Mental Hlth; Universidade Federal de São Paulo (UNIFESP)Recent indications of oxidative stress in hypothalamus of sleep deprived rats prompted us to address the possibility that sleep deprivation may induce pathological cell loss changes in brain. Indices of necrosis and apoptosis were quantified after 96 h of sleep deprivation induced by the classical platform technique in rats. Binding of the peripheral-type benzodiazepine ligand [H-3] PK 11195 to reactive astrocytes, a reliable and sensitive index of necrotic changes, was not altered in any of 14 brain regions examined. Likewise, no changes were found in mRNA levels of the apoptosis-related genes bcl-2 and bax in any of 24 brain regions examined. This was corroborated by quantitative TUNEL analyses in hypothalamus, amygdala, and cortex, which also revealed no effects in sleep deprived animals. These results are consistent with other recent evidence that sleep deprivation does not induce necrotic or apoptotic cell loss in brain. This suggests that recent findings of oxidative stress in sleep deprived brains do not result in cell loss. the possibility that sleep deprivation may result in functional deficits, or that structural changes may emerge after repeated episodes of sleep deprivation, remains to be addressed.