Navegando por Palavras-chave "Unilateral ureteral obstruction"
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- ItemSomente MetadadadosDeletion of bradykinin B1 receptor reduces renal fibrosis(Elsevier B.V., 2009-06-01) Wang, Pamella Huey Mei [UNIFESP]; Cenedeze, Marcos Antonio [UNIFESP]; Campanholle, Gabriela [UNIFESP]; Malheiros, Denise Maria Avancini Costa; Moura Torres, Hugo Arruda de [UNIFESP]; Pesquero, João Bosco [UNIFESP]; Pacheco-Silva, Alvaro [UNIFESP]; Saraiva Camara, Niels Olsen [UNIFESP]; Universidade Federal de São Paulo (UNIFESP)The Kallikrein-kinin system works through activation of two receptors. One constitutive, named B2 receptor (B2R) and another inducible, denominated 131 receptor (B1R). in renal fibrosis, B2R receptor activation appears to be protective, however B1R participation is unveiled. the aim of this study was to analyze how the deletion of the Ell R would modify tissue responses after unilateral ureteral obstruction (UUO). for that, B1R knockout (131 KO) and wild-type mice (B1B2WT) were subjected to UUO and sacrificed at days 1, 5 and 14. Renal dysfunction was assayed by urine proteinuria/creatinine ratio and percentage of tubulointerstitial fibrosis. Kidneys were harvested at day 5 to analyze anti and pro-inflammatory molecules expression by real-time PCR. We demonstrated that at all time points, B1KO mice presented lower proteinuria/creatinine ratio from bladder urine. Ell KO protection was reinforced by its lower tubular interstitial fibrosis percentage at day 14 (B1B2WT: 12.16 +/- 1.53% vs. B1KO: 6.73 +/- 1.07%, p<0.02). UUO was able to induce B1R expression and its highest transcription was achieved at day 5. At this day, B1KO had significant lower expression of proinflammatory molecules such as TGF-beta, MCP-1, OPN and IL-6 and higher anti-inflammatory components, as IL-10 and HO-1. Herein, we observed that Ell R deletion may be an important component in renal fibrosis prevention. (C) 2008 Elsevier B.V. All rights reserved.
- ItemAcesso aberto (Open Access)Efeito da suplementação com ácidos graxos ômega 3 (ω-3) nas propriedades das células estromais mesenquimais da medula óssea(Universidade Federal de São Paulo (UNIFESP), 2018-12-20) Oliveira, Andreia Silva de [UNIFESP]; Borges, Fernanda Teixeira [UNIFESP]; Schor, Nestor [UNIFESP]; http://lattes.cnpq.br/8276708741672261; http://lattes.cnpq.br/4206613998602417; http://lattes.cnpq.br/4123390663752858; Universidade Federal de São Paulo (UNIFESP)Mesenchymal stromal cells (MSCs) are characterized by the ability to differentiate into mesoderm cells, multiplication to maintain their undifferentiated state and expression of surface markers such as CD90 and CD73, but not CD45, in addition to others. They have ability to target the injured site and present antiinflammatories, antioxidants and immunosuppressants effects. Donor characteristics may influence the characteristics and / or effects of MSCs. This study aimed to perform dietary supplementation with omega3 polyunsaturated fatty acids in the characteristics of mesenchymal stromal cells. Wistar rats were supplemented for 28 days with eicosatetraenoic acid (EPA) and docosahexaenoic acid (DHA) (2mg/kg body weight), as MSCs were harvested, cultured and analyzed in the 4th passage (MSCs3) and compared with animal cells not supplemented (MSCs). The capacity of selfrenewal, adipogenic and osteogenic differentiation, response to hypoxia and repairing effect in a unilateral ureteral obstruction (UUO) model were analyzed. Differences in morphology and in the differentiation capacity between MSCs and MSCs3 was not observed. The latter has small capacity for selfrenewal, but both showed the same apoptotic rates under hypoxic conditions. In severe chronic diseases induced by UUO, we observed that as MSCs3 aggravated an infiltration of immune cells in relation to obstructed kidneys. In the transplanted kidneys with MSCs3, there was greater expression of superoxide dismutase1, IL10, and less IL6 production. There was no significant fibrosis in the obstructed kidneys, but there were increased levels of TGFβ1 in the UUO group. It decreased in those transplanted with MSCs3, suggesting a longterm antifibrotic effect as observed in MSCs. Our results suggest that supplementation of omega3 donors may interfere with the characteristics and effects of MSCs and supplementation should be taken into account when using these cells for therapy.
- ItemSomente MetadadadosEndostatin, an antiangiogenic protein, is expressed in the unilateral ureteral obstruction mice model(Wichtig Editore, 2008-09-01) Maciel, Thiago T. [UNIFESP]; Coutinho, Enia L. [UNIFESP]; Soares, Debora [UNIFESP]; Achar, Eduardo [UNIFESP]; Schor, Nestor [UNIFESP]; Bellini, Maria H. [UNIFESP]; Universidade Federal de São Paulo (UNIFESP); IPEN CNEN SPBackground: Extracellular matrix accumulation, epithelial-to-mesenchymal transition, tubular atrophy and loss of peritubular capillary network are hallmarks of tubulointerstitial injury in progressive renal diseases. In this study, we analyzed endostatin expression in kidneys subjected to unilateral ureteral obstruction (UUO).Methods: Collagen XVIII mRNA expression was evaluated by real-time polymerase chain reaction (PCR). Endostatin and CD31 protein levels were analyzed by Western blot and immunohistochemistry. In vitro quantification of collagen XVIII and fibrosis-related genes in HK2 cells was performed by real-time PCR.Results: UUO significantly increased collagen XVIII mRNA expression and released a 30-kDa endostatin fragment. Immunohistochemistry revealed endostatin expression increased in injured tissue, mainly on tubular cells. Of interest, expression of CD31 was significantly reduced by UUO. Endostatin administration in vitro did not modify the expression of genes related to fibrosis development. However, in vitro TGF-b1 administration induced expression of collagen XVIII/endostatin mRNA in human tubular cells.Conclusion: Endostatin is expressed during the progression of renal fibrosis in vitro and in vivo, suggesting a role for endostatin in development of tubulointerstitial injury.